中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (5): 312-314.

• 论著 • 上一篇    下一篇

红色毛癣菌菌落产色变异的实验研究

刘维达1, 王金燕2, 吕桂霞1, 吴建明3   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042;
    2. 内蒙古医学院附属第一医院皮肤科;
    3. 江苏建湖县皮防所
  • 收稿日期:1997-04-05 修回日期:1997-06-18 出版日期:1997-10-15 发布日期:1997-10-15

Study of the Variation of Formation of Red Color on Colony for T.rubrum

Liu Weida1, Wang Jinyan2, Lü Guixia1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1997-04-05 Revised:1997-06-18 Online:1997-10-15 Published:1997-10-15

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摘要： 红色毛癣菌菌落产生红色是该菌重要鉴定特征之一,但常遇到不产生红色的菌株.了解这种产色变异的范围和频率以及影响因素,对提高鉴定正确性、更好地指导治疗有重要意义.方法:对200株不同地区来源的临床株进行初代分离和传代培养,并观察1%葡萄糖玉米粉吐温琼脂和马铃薯葡萄糖琼脂对产色的恢复作用;比较不同培养条件对产色变异的影响,对有关数据进行统计学处理.结果:初代产色消失率为34%,传代诱导消失率为49%,稳定消失率达24%.大多数传代消失为可逆的,而大多数初代变异为不可逆的;较高的pH和较低的培养温度不利于产色.北方分离株产色消失率显著高于南方分离株.结论:红色毛癣菌产色消失率发生较高,已对其鉴定产生影响,其确切的发生机理,有待深入研究.

关键词: 红色毛癣菌, 产色消失, 变异

Abstract: Purpose:The formation of red color on the colony of T.rubrum is an important character for the identification of the fungus.But,those strains which cannot yield red color on the colony are isolated frequently.It is important to investigate the range,frequency and influence factors of the color variation of T.rubrum for the identification of the fungus and directing therapy.Method:200 strains of T.rubrum from different areas of China were isolated as the first generation and cultured serially.The resumptive actions of culture media (DCTA and PDA) on yielding color were observed,and the influences of different culture conditions on the color variation were compared.Result:The color variation rate of the first generation was 34% in 200 strains tested.The color variation rate induced by serial cultures was 49%.The majority variations induced by serial cultures were reversible,but the majority variations of the first generation were irreversible.Higher pH value of the cultures and lower cultural temperature were unfavorable in the formation of red color.The color variation rate of isolates from the North China was markedly higher than those from the South China.Conclusion:The frequency of color variation of T.rubrum is high that influences the identification of the fungus.The definite mechanism of the color variation remains to be studied.

Key words: Trichophyton rubrum, Phenotype, Variation