中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (5): 299-301.

• 论著 • 上一篇    下一篇

裴氏着色真菌基因组DNA文库的构建

张宏1, 吴绍熙1, 郭宁如1, 徐贤秀2, 周爱武2   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042;
    2. 南京大学医药与生物技术国家重点实验室
  • 收稿日期:1996-11-28 修回日期:1997-05-08 出版日期:1997-10-15 发布日期:1997-10-15

Construction of Fonsecaea pedrosoi Genomic DNA Library

Zhang Hong1, Wu Shaoxi1, Guo Ningru1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1996-11-28 Revised:1997-05-08 Online:1997-10-15 Published:1997-10-15

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摘要： 为了进一步研究裴氏着色真菌基因组DNA组成,以便为快速、准确地鉴定和诊断该菌的感染提供依据,采用Sau3AI部分酶切基因组DNA,以pUC18为载体,首次成功地构建了裴氏着色真菌基因组DNA文库.插入片段的长度在2.5～6.0kb,DNA文库的完整性在84%以上.结果提示本文库大小适度、完整性较高,在实验诊断、菌种鉴定和探针筛选等方面有应用潜力.

关键词: 裴氏着色真菌, DNA文库

Abstract: For more accurate and simple identification of Fonsecaea pedrosoi based on genomic composition in clinical and experimental mycological laboratories,we report the first construction of F.pedrosoi genomic DNA library in plasmid pUC18 vector by using the insertion of Sau 3AI partially digested fragments 2.5 to 6.0kb in length.The exact probability of having any DNA sequence represented in the library is above 84%.It is suggested that the library will be of value for the identification and mycological diagnosis of F.pedrosoi infection in the future,and be useful as the basis for screening a series of genomic DNA probes.

Key words: Fonsecaea pedrosoi, DNA library