中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (3): 178-181.

• 论著 • 上一篇    下一篇

扫描电镜下梅毒螺旋体超微结构及其对宿主细胞表面的损害

王建华   

  1. 深圳市红十字会医院皮肤性病科, 518029
  • 收稿日期:1996-04-27 修回日期:1997-01-29 出版日期:1997-06-15 发布日期:1997-06-15

Ultrastructure of Treponema Pallidum and Host Cell Surface Damage Caused by Treponema Pallidum under Scanning Electron Microscope

Wang Jianhua   

  1. Department of Dermatovenereology, Shenzhen Red Cross Hospital, Shenzhen 518029
  • Received:1996-04-27 Revised:1997-01-29 Online:1997-06-15 Published:1997-06-15

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摘要： 为研究梅毒螺旋体超微结构及其对宿主细胞表面的损伤作用,对13例早期梅毒患者扁平湿疣损害的上皮细胞进行冷冻割断处理,应用扫描电镜对其进行了观察。在扫描电镜下:每条梅毒螺旋体呈7～12个螺旋,每个螺旋的投影波长为0.6～1.2μm,振幅0.15～0.3μm.梅毒螺旋体两端轴长7.0～13.0μm,其在扫描电镜下投影的体轴为8.3～22.0μm,梅毒螺旋体两端的轴长与体轴之比为1:1.2～1.9.梅毒螺旋体横断面直径约0.16μm.在经冷冻割断处理的宿主细胞表面可见粘附着大量的梅毒螺旋体,在一幅扫描电镜观察面见宿主细胞胞膜附着20多条梅毒螺旋体。粘附梅毒螺旋体的宿主细胞胞膜见大小不等的小孔。梅毒螺旋体粘附宿主细胞表面,使得宿主细胞膜被破坏,由此产生一系列组织和细胞的病理变化。

关键词: 梅毒螺旋体, 扫描电镜, 粘附, 宿主细胞

Abstract: In order to study the ultrastructure of T.pallidum and damaging effect of it to host cell surface,the epithelial cells of 13 patients with condyloma latum were frozen and cut,and the epithelial cell surface was examined with the scanning electron microscope(SEM). Under SEM, each T.pallidum had 7 to 12 spirals. The projection wave length of the T.pallidum was 0.6 to 1.2μm, and the amplitude was 0.15 to 0.3μm. The length of two terminal axes of T.pallidum was 7.0 to 13.0μm, and the projection body axis was 8.3 to 22.0μm in length, while the proportion of the two terminal axes and body axis was 1:1.2 to 1.9. The diameter of transverse section of T.pallidum was about 0.16μm. A large amount of T.pallidum were seen adhered to one host cell surface after freezing and cutting treatment. On one image of the SEM,more than 20 T.pallidum were seen adhering to the host cell mambrane.On the host cell membrane adhered with T.pallidum, holes of different sizes could be seen. The results suggest that after adhering of T.pallidum to the host cell surface, the host cell membrane is damaged, and causes a series of pathological changes of tissue and cells.

Key words: Treponema pallidum, Scanning electron microscope, Adhere, Host cell