中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (5): 346-348.

• 论著 • 上一篇    下一篇

T细胞受体基因重排检测皮肤T细胞淋巴瘤

张春雷1, 宫恩聪2, 刘永生3, 沈力1, 李世荫1   

  1. 1. 北京医科大学第三临床医学院皮肤科, 100083;
    2. 北京医科大学第三临床医学院病理学系, 100083;
    3. 中日友好医院皮肤科
  • 收稿日期:1995-09-15 修回日期:1996-01-30 出版日期:1996-10-15 发布日期:1996-10-15

Study on Clinical Application of Detection of T-cell Receptor Gene Rearrangement in Cutaneous T-cell Lymphoma

Zhang Chunlei1, Gong Encong2, Liu Yongsheng3   

  1. Department of Dermatology, The Third Hospital, Beijing Medical University, Beijing 100083
  • Received:1995-09-15 Revised:1996-01-30 Online:1996-10-15 Published:1996-10-15

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摘要： 为了研究基因诊断在皮肤T细胞淋巴瘤(CTCL)的临床应用,采用聚合酶链反应(PCR)技术,对60例皮肤淋巴细胞浸润疾患进行了T细胞受体(TCR)基因重排的检测。结果显示,检出TCR-β和(或)TCR-γ基因克隆重排的有:36/40例CTCL、4/6例可疑蕈样肉芽肿/Sézary综合征和1/1例淋巴瘤样丘疹病;未检出TCR-β或γ基因克隆重排的有:2例皮肤B细胞淋巴瘤、1例皮肤淋巴细胞瘤、8例良性皮肤淋巴细胞浸润疾患和2例正常人皮肤。这表明:该方法在确定CTCL的克隆性、细胞源性以及早期诊断方面是有帮助的。

关键词: 皮肤T细胞淋巴瘤, T细胞受体, 聚合酶链反应

Abstract: To study the clinical application of molecular diagnosis in cutaneous T-cell lymphoma (CTCL),the polymerase chain reaction Was used to detect the rearrangements of T-cell receptor (TCR)gene in 60 patients with cutaneous lymphocytic infiltrates.The result showed that dominant clonal TCR-β or γ gene rearrangements were detected in 36/40 CTCL,4/6 suspected mycosis fungoldes/Sézary syndrome,1/1 lymphomatoid papulosis.No dominant clonal TCR-β or γ gene rearrangements were detected in 2 patients with CBCL,1 patient with lymphocytoma cutis,8 patient with benign lymphocytic infiltrates of skin and 2 normal controls.This study demonstrates that the method is helpful for the lineage determination,clonality and early diagnosis of CTCL.

Key words: Cutaneous T-cell Lymphoma, T-cell receptor, PCR