中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (5): 339-342.

• 论著 • 上一篇    下一篇

须癣毛癣菌随机扩增DNA多态性分型研究

钟志红, 李若瑜, 李冬梅, 王端礼   

  1. 1. 中国科学院微生物所真菌与地衣系统学开放实验室, 100080;
    2. 北京医科大学第一医院皮肤科, 100034
  • 收稿日期:1996-03-18 修回日期:1996-06-05 出版日期:1996-10-15 发布日期:1996-10-15

Typing of Trichophyton mentagrophytes by Random Amplification of Polymorphic DNA

Zhong Zhihong, Li Ruoyu, Li Dongmei   

  1. Department of Dermatology, The First Hospital, Beijing Medical University, Beijing 100034
  • Received:1996-03-18 Revised:1996-06-05 Online:1996-10-15 Published:1996-10-15

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摘要： 研究须癣毛癣菌的DNA分型,了解DNA型别与形态学分型、有性型型别,以及与菌种来源地区、与人体感染部位之间的关系。用氯化苄法提取DNA,以随机扩增DNA多态性方法对临床分离的须癣毛癣菌42株以及部分其它皮肤癣菌和我科保藏的菌种Arthroderma 8株进行分型。结果:①42株临床分离的须癣毛癣菌可分为三个DNA型,某些引物的扩增还可体现出型内菌株间的多态性;②须癣毛癣菌的DNA型与形态学分型之间并无简单的对应关系,而与其来源地区有密切的联系,不同取材部位的菌株DNA型亦有一定特点;③有性型A.benhamiae,A.vanbreuseghemii的DNA型各不相同,而42株临床分离的须癣毛癣菌均具有与A.vanbreuseghemii相似的DNA带型。结论:随机扩增DNA多态性将临床分离的须癣毛癣菌分为3型,其DNA型与形态学、有性型,以及与来源地区、与人体感染部位之间存在一定联系。本研究对于病原学诊断、判断感染来源、了解菌种地区分布等流行病学特点具有重要的意义。

关键词: 须癣毛癣菌, DNA分型, 随机扩增DNA多态性

Abstract: To investigate the DNA typing of Trichophyton mentagrophytes,and to find the relationship of DNA type with morphology,teleomorph type,the geographic origin and the sites of infection. DNA extraction was carried out by using benzyl chloride and random amplification of polymorphic DNA (RAPD)assay was employed for DNA typing of 42 strains of T.mentagrophytes isolated from clinical specimens and some other dermatophytes,also 8 preserved strains of Arthroderma.Results:(1)Forty two isolates of T.mentagrophytes can be classified into 3 main types,and the intratype polymorphism has been revealed with some primers.(2)There is no simple relationship between DNA type and morphology,but the DNA types are closely related to the geographic origin,and the isolates from different sites of infection have their own genotype characteristics.(3)DNA patterns of Arthroderma benhamiae and A.vanbreuseghemii are distinctly different,while 42 clinical isolates of T.rnentagrophytes share similar pattern with A.vanbreuseghemii.The clinical isolates of T.mentagrophytes can be divided into 3 types by RAPD,and its DNA type has certain relationship with morphology,teleomorph type,the geographic origin and ttie sites of infection.RAPD provides an useful tool for the pathogenic diagnosis and epidemiology study to investigate the source of infection,and geographic distribution of strains.

Key words: Trichophyton mentagrophytes, DNA typing, Random amplification of polymorphie DNA