中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (5): 335-338.

• 论著 • 上一篇    下一篇

聚维酮碘溶液抗白念珠菌的实验研究

冉玉平1, 张朝武2, 坪井良治3, 小川秀兴3   

  1. 1. 华西医科大学附属第一医院皮肤科, 成都610041;
    2. 华西医科大学公共卫生学院;
    3. 日本顺天堂大学医学部皮肤科
  • 收稿日期:1996-04-02 修回日期:1996-06-18 出版日期:1996-10-15 发布日期:1996-10-15

Experimental Study of Antifungal Effect of Povidone Iodine Solution on Candida Albicans

Ran Yuping1, Zhang Chaowu2, Tsuboi Rioji3   

  1. Department of Dermatology, The First Hospital, West China University of Medical Sciences, Chengdu 610041
  • Received:1996-04-02 Revised:1996-06-18 Online:1996-10-15 Published:1996-10-15

摘要: 研究聚维酮碘溶液对白念珠菌代谢活性及经聚维酮碘溶液处理的白念珠菌对培养人角朊细胞的影响。采用alamar Blue荧光分析法测得聚维酮碘溶液浓度依赖性抑制白念珠菌代谢活性,LD50为0.075%。经2%聚维酮碘溶液/PBS(一)处理30分钟的白念珠菌几乎失去对培养角朊细胞的粘附和侵袭作用;孢子表面凹凸不平,明显皱缩,细胞内结构紊乱,不能发育成菌丝。经2%聚维酮碘溶液/PBS(一)处理30分钟和10%聚维酮碘溶液/沙堡液体培养基处理33小时后的白念珠菌转种到沙堡葡萄糖琼脂上不能长出菌落,提示在不同溶剂条件下聚维酮碘溶液的杀白念珠菌效果有差异。

关键词: 聚维酮碘, 白念珠菌, 角朊细胞

Abstract: The effect of povidone iodine solution(PIS)on the metabolic activity of Candida albicans and the effect of PIS treated C.albicans to cultured human keratinocytes were studied by using the Alamar blue fluorescence assay.It was found that PIS inhibited the metabolic activity of Candida albicans with a dose-dependent manner,and that the LD50 of PIS for Candida albicans is 0.075%.After treating for 30 min by 2% PIS/PBS(-),Candida albicans almost lost the adherence and invasion to the cultured human keratinocytes,the wall of yeast cells became uneven and shrunken,the intracellular structures became obviously indistinct,and the yeasts could not develop to hypha form.Candida albicans,treated with 2% PIS in PBS(-)for 30 min or with 10% PIS Sabouraud's liquid medium for 33 hr,did not form colony when it was inoculated to Sabouraud's dextrose agar.This result suggests that the fungicidal effective of PIS is influenced by its solvent.

Key words: Povidone iodine, Candida albieans, Keratinocytes