中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (5): 327-329.

• 论著 • 上一篇    下一篇

白念珠菌蛋白酶对中性粒细胞和淋巴细胞的趋化性研究

冉玉平1, 坪井良治2, 岩渊和久3, 长冈功3, 小川秀兴2   

  1. 1. 华西医科大学附属第一医院皮肤科, 成都610041;
    2. 日本顺天堂大学医学部皮肤科;
    3. 日本顺天堂大学医学部皮肤科第二生化
  • 收稿日期:1996-01-23 修回日期:1996-05-28 出版日期:1996-10-15 发布日期:1996-10-15

Study on Chemotactic activity of Candida Albicans Proteinase for Neutrophils and Lymphocytes

Ran Yuping1, Tsuboi Ryoji2, Iwabuchi Kazuhisa3   

  1. Department of Dermatology, The First Hospital, West China University of MedicalSciences, Chengdu 610041
  • Received:1996-01-23 Revised:1996-05-28 Online:1996-10-15 Published:1996-10-15

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摘要： 采用改良Boyden室法测定了白念珠菌蛋白酶(Candida albicans proteinase,CAP)对人末梢血白细胞的趋化活性。发现CAP对中性粒细胞的趋化活性随浓度增高而增强,在500nmol/L时达到最大值(P<0.001)。100℃处理CAP10分钟后趋化活性显著降低。棋盘法分析发现CAP对中性粒细胞不仅有趋化活性,而且有化学激活性。CAP仅引起淋巴细胞轻微趋化。在3只豚鼠真皮内注射CAP,3小时后的活检标本中发现真皮血管周围的中性粒细胞平均浸润数为29个/视野,显著多于注射磷酸盐缓冲液的平均3个/视野(P<0.05)。此结果提示,作为蛋白水解酶的CAP不仅与菌体侵入机体和增殖有关,同时可诱导末梢血中性粒细胞向感染局部移动。

关键词: 白念珠菌, 蛋白酶, 趋化性, 中性粒细胞, 淋巴细胞

Abstract: The chemotactic activity of Candida albicans proteinase toward human peripheral leukocytes was examined by a modified Boyden chamber method.It was found that C.albicans proteinase induce dose-dependent chemotactic activity for neutrophils with maximal activity at 500 nmol/L(P< 0.001).Chemotactic activity was significantly reduced after 10 min treatment at 100℃.Checkerboard analysis indicated that C.albicans proteinase is chemotactic and activates neutrphils chemically.Mild chemotactic activity was observed for peripheral lymphocytes.C.albicans proteinase injected intraderreally into the skin of 3 guinea pigs,it induced significantly more infiltration of neutrophils(29 cells/field)around the dermal vessels than did phosphate buffered saline(3 cells/field,P<0.05)at the 3hour biopsy samples.These results suggest that C.albicans proteinase,which theoretically acts as a hydrolase for fungal invasion and growth,induces the migration of peripheral neutrophils to the C.albicans-infected site.

Key words: Candida albicans, Proteinase, Chemotaxis, Neutrophil, Lymphocyte