中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (5): 322-323.

• 论著 • 上一篇    下一篇

从芦苇中分离孢子丝菌的研究

张民夫, 张亚芹, 姜萍, 段正芳, 杨国际   

  1. 白求恩医科大学第二临床学院皮肤科, 长春130041
  • 收稿日期:1995-12-26 修回日期:1996-05-08 出版日期:1996-10-15 发布日期:1996-10-15

Study on Isolating Sporothrix Schenckii from Reed

Zhang Minfu, Zhang Yaqin, Jiang Ping   

  1. Department of Dermatology, Second Clinical College, N.Bethune University of Medical Sciences, Changchun 130041
  • Received:1995-12-26 Revised:1996-05-08 Online:1996-10-15 Published:1996-10-15

摘要: 吉林省通榆县是孢子丝菌病高发地区。为了从自然环境中分离出孢子丝菌病的病原菌,我们采集吉林省通榆县造纸厂芦苇垛底部腐烂的芦苇,按真菌学方法分离培养,对初代和二代菌落的形态、结构进行了观察。对菌组织做了ABC-ELISA检测以验证其免疫学特异性,并做了动物实验,对其致病性及病理改变进行了观察。研究结果证实从芦苇中分离出申克孢子丝菌。结论:孢子丝菌病发病率高的地区腐烂的芦苇中有申克孢子丝菌寄生。

关键词: 孢子丝菌病, 孢子丝菌, 芦苇

Abstract: On the basis of epidemiologic survey we have identified that Tongyu county of Jilin provice is a region with high incidence of sporotrichosis.In order to isolalate the pathogenic fungus of sporotrichosis from natural environment,we collected the decayed reed from the bottom of the reed pile at the papermill located in the county,then cultured the sample of the reed and isolated the fungi found.The morphology of colony and fungus structure isolated were observed for the first and secondary colony.The immunological characteristics were studied by ABC-ELISA,and the pathogenicity of the isolates and histopathological changes were studied in experimental animals.The results verify that the fungi isolated from reed were Sporothrix schenckii.The conclusion of study shows there are parasitic Sporothrix schenckii in the putrid reed in the region with high incidence rate of sporotrichosis.

Key words: Sporotrichosis, Sporothrix schenckii, Reed