中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (5): 319-321.

• 论著 • 上一篇    下一篇

聚合酶链反应快速诊断隐球菌性脑膜炎

顾菊林1, 廖万清1, 柴建华2   

  1. 1. 第二军医大学长征医院皮肤科, 中国医学真菌保藏管理中心隐球菌专业实验室, 上海200003;
    2. 复旦大学遗传学研究所
  • 收稿日期:1995-07-09 修回日期:1995-11-08 出版日期:1996-10-15 发布日期:1996-10-15
  • 基金资助:
    中华医学会皮肤科学会科研基金赞助项目

Rapid Diagnosis of Oryptococcal Meningitis by Polymerase Chain Reaction

Gu Julin1, Liao Wanqing1, Chai Jianhua2   

  1. Department of Dermatology, Changzheng Hospital, Shanghai 200003
  • Received:1995-07-09 Revised:1995-11-08 Online:1996-10-15 Published:1996-10-15

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摘要： 在国内首次采用聚合酶链反应扩增新生隐球菌rDNA部分特异性序列的方法诊断隐球菌性脑膜炎。所试11株新生隐球菌均扩增产生预期长度136bp的特异性片断,21株非新生隐球菌未见此片断。扩增灵敏度为10cfu。临床标本的PCR检测结果为:23份涂片法或(和)真菌培养阳性的脑脊液标本扩增也均为阳性(100%),14份涂片法和真菌培养阴性的脑脊液标本13份扩增阴性(93%)。认为rDNA聚合酶链反应是诊断隐球菌性脑膜炎的一种简便快速、灵敏、特异的方法。

关键词: 隐球菌性脑膜炎, 聚合酶链反应, rDNA

Abstract: Polymerase chain reaction(PCR) was used for the first time in China for the diagnosis of cryptococcal meningitis by amplification of specific sequence of the rDNA genes of C.neoformans.All 11 strains of C.neoformans yielded a specific 136 bp fragment but 21 non-C.neoformans strains did not. The sensitivity of the amplification was about 10 cfu.With the aid of the rDNA-PCR,23 of the 23 cerebrospinal fluids (CSF) specimens which had been confirmed C.neoforrnans positive by smear and/or culture were PCR-positive(100%),and 13 of the 14 CSF specimens which had been confirmed C.neoformans negative by smear and culture were PCR-negative (93%).The results of the present study suggest that rDNA-PCR is a sensitive and specific method for rapid diagnosis of cryptococcal meningitis.

Key words: Cryptococcal meningitis, PCR, rDNA