中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (3): 183-185.

• 论著 • 上一篇    下一篇

几种皮肤病中角朊细胞表达ICAM-1(CD54)和HLA-DR抗原的研究

何春涤, 王雅坤, 陈洪铎   

  1. 中国医科大学第一临床学院皮肤科 沈阳 110001
  • 收稿日期:1995-08-22 修回日期:1995-12-07 出版日期:1996-06-15 发布日期:1996-06-15
  • 基金资助:
    国家自然科学基金

Expression of ICAM-1(CD54) and HLA-DR Antigens on Keratinocytes in Some Dermatoses

He Chun-di, Wang Yakun, Chen Hongduo   

  1. Department of Dermatology, No.1 Hospital of China Medical University, Shenyang 110001
  • Received:1995-08-22 Revised:1995-12-07 Online:1996-06-15 Published:1996-06-15

摘要: 为了探讨皮肤病病损处角朊细胞表达ICAM-1(CD54)和HLA-DR抗原及其与病损内浸润细胞的关系,应用抗ICAM-1及HLA-DR单抗、连续冰冻切片法及ABC免疫组化技术,在寻常型银屑病(4/4)、扁平苔藓(5/6)、慢性湿疹(4/6)、孢子丝菌病(3/6)、基底细胞癌(6/6)、鳞状细胞癌(5/7)、及Ⅱ期(4/5)、Ⅲ期(1/4)蕈样肉芽肿中,观察到其病损处角朊细胞可分别表达CD54和HLA-DR抗原.我们还发现在部分扁平苔藓(1/6)、慢性湿疹(2/6)、孢子丝菌病(3/6)、鳞状细胞癌(2/7)及Ⅱ期(1/5)、Ⅲ期(1/4)蕈样肉芽肿中,虽然其病损处真皮内见大量的T淋巴细胞浸润,但其上方表皮内角朊细胞未见CD54抗原表达,却可见HLA-DR抗原表达.在蕈样肉芽肿中,随病程进展到Ⅲ期(2/4),其病损处角朊细胞不仅有失去表达CD54、同时也有失去表达HLA-DR抗原能力的趋势.并探讨了病损内浸润细胞免疫表型与角朊细胞表达CD54抗原的关系.

关键词: 角朊细胞, 抗体, 单克隆, 细胞间粘连分子-1

Abstract: In order to investigate the expresion of ICAM-1(CD54) and HLA-DR antigens on lesional keratinocytes(KCs) in some dermatoses,and its relationship with the dermal infiltrates in lesions,the ICAM-1 and HLA-DRMAbs,serial sections of frozen biopsy specimens and ABC immunoper-oxidase technique were employed.The expresion of CD54 and HLA-DR antigens on lesional KCs could be observed respectively in most specimens of psoriasis vulgaris(4/4,denominat or indicates the No.of the cases examined and the numerat or the No.of positive ones,the same below),lichen planus(5/6),chronic eczema(4/6),sporotrichosis(3/6),basal cell carcinoma(6/6),squamous cell carcinoma(5/7),and mycosis fungoides(MF) stage Ⅱ(4/5),stageⅢ(1/4).

Key words: Keratinocytes, Antibodies,monoclonal, ICAM-1