中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (3): 169-171.

• 论著 • 上一篇    下一篇

生殖器疱疹的诊断及感染特征探讨

白桦, 佟菊贞, 叶兴东, 李欢, 涂裕英, 王树兴   

  1. 中山医科大学第一附属医院皮肤科 广州 510089
  • 收稿日期:1995-07-18 修回日期:1996-03-18 出版日期:1996-06-15 发布日期:1996-06-15
  • 基金资助:
    国家自然科学基金

Study on Diagnosis and Clinical Features of Genital Herpes

Bai Hua, Tong Juzen, Ye Xingdong   

  1. The First Afiliated Hospital, Sun Yat-San University of Medical Sciences, Guangzhou 510089
  • Received:1995-07-18 Revised:1996-03-18 Online:1996-06-15 Published:1996-06-15

摘要: 细胞培养法对80例临床诊断为典型生殖器疱疹的患者进行了人类单纯疱疹病毒(HSV)分离,并结合链霉亲和素过氧化物酶免疫组化法(LSAB)及PCR法对其中部分患者进行了HSV及其型别检查.三种方法的HSV检出率分别为58.8%、20%、90%;培养法作为金标准特异性高,对早期患者检出率达86.4%;PCR法与其他两法比较敏感性高,并利于HSV型别的诊断;LSAB法对临床标本的检出率虽很低,却可提高培养法的检出率.HSV的感染型别HSV2占91.7%、HSV1占8.3%.还讨论了PCR引物的优点,并总结了本组患者的临床感染特征.

关键词: 生殖器疱疹, Vero细胞, LSAB, PCR

Abstract: Herpes simple xvirus(HSV) was isolated in 80 patients with typical genital herpes using Vero celmethod,and in apart of these patients HSV was detected and typed by using LSAB and PCR.The detection rates of cell culture,LSAB and PCR were 58.8%,20%,and 90% respectively.PCR was good for HSV typing in addition.The positive rate of HSV-2 was 91.7%,HSV-1 was 8.3%.LSAB showed lower sensitivity in clinical specimens,but higher detection rate in cell culture specimens.The advantage of the primers used and clinical features in this study were discussed also.

Key words: Genital herpes, Vero cell, LSAB, PCR