中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (3): 157-160.

• 论著 • 上一篇    下一篇

人类免疫缺陷病毒感染患者中淋巴因子基因表达的动态变化

范江1, 陈若延1, Thang Nguyen2, John L. Fahey2   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042;
    2. 美国加州大学洛山矶分校医学院
  • 收稿日期:1995-02-20 修回日期:1995-07-13 出版日期:1996-06-15 发布日期:1996-06-15

The Kinetics of Cytokine Gene Expression in PBMC from HIV Seropositive Subjects Stimulated by Phy-tohaemagglutinin,Pokeweed Mitogen,Candidin,Anti-CD 3 Monoclonal Antibody and Recombinant IL-2

Fan Jiang1, Chen Ruoyian1, Thang Nguyen2   

  1. 1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical Colege, Nanjing 210042;
    2. UCLA, School of Medicine, Los Angeles, USA
  • Received:1995-02-20 Revised:1995-07-13 Online:1996-06-15 Published:1996-06-15

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摘要： 在感染性疾病中,淋巴因子与免疫调节机制密切相关.为了解在人类免疫缺陷病毒(HIV)感染患者中淋巴因子对多种刺激原的动态变化反应情况,以便进一步弄清HIV感染的病理变化机制,我们采用定量逆转录聚合酶链反应的方法(RT-PCR),对经植物血凝素(PHA)、商陆致丝裂素(PWM)、念珠菌蛋白(candidin)、抗CD3单克隆抗体(Anti-CD3MAb)和重组白介素2(rIL2)刺激后的HIV感染者及对照组外周血单一核细胞(PBMC)中的白介素2(IL2)、γ干扰素(IFN-γ)、白介素10(IL10)、白介素6(IL6)、肿瘤坏死因子α(TNF-α)和IL2受体(IL2R)的动态变化进行了比较.在HIV感染组中,其IL2和IL2R基因表达水平明显低于对照组.IL2和IFN-γ均由T辅助细胞一型(TH1)克隆产生,然而在HIV感染患者中,IFN-γ的水平却明显增高.

关键词: HIV, 淋巴因子, 刺激原

Abstract: Cytokines were closely related to the major immunoregulatory mechanism in infectious disease.The kinetics of cytokine gene expressions including IL-2,IFN-γ,IL-10,IL-6,TNF-α and IL-2 receptor in PBMC from HIV seropositive and seronegative subjects were compared by quantitative reverse transcription polymerase chain reaction studies stimulated by phytohaemaglutinin(PHA),pokewed mitogen(PWM),candidin,anti-CD3 monoclonal antibody (Anti-CD3m Ab) and recombinant IL-2(rIL-2).IL-2 and IL-2 recept or (IL-2R) to PHA,PWM,candidin,anti-CD3 mAb and rIL-2 stimulation were markedly lowered in patients with HIV infection.

Key words: HIV, Cytokine, Stimulation