Langerhans细胞组织细胞增生症的免疫组化电镜及DNA流式细胞计数研究

中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (4): 222-225.

Langerhans细胞组织细胞增生症的免疫组化电镜及DNA流式细胞计数研究

王健美¹, 唐曙², 陈仁贵², 凌波²

1. 上海第二医科大学附属新华医院皮肤科 200092;
2. 华北煤炭医学院附属医院皮肤科 063000

收稿日期:1994-07-20 修回日期:1995-02-06 出版日期:1995-08-15 发布日期:1995-08-15

Immunohistochemical, Ultrastructural Studies and Flow Cytometric DNA Analysis in Langerhans Cell Histiocytosis

Wang Jianmei¹, Tang Shu², Chen Rengui²

Department of Dermatology, Xin-Hua Hospital, Shanghai Second Medical University, Shanghai 200092

Received:1994-07-20 Revised:1995-02-06 Online:1995-08-15 Published:1995-08-15

摘要/Abstract

摘要： 免疫组化、电镜及流式细胞计数对15例Langerhans细胞组织细胞增生症的皮肤活检标本进行了研究。15例中13例免疫组化染色对抗S100蛋白、花生凝集素反应阳性,对抗Mac387和溶菌酶反应阴性;3例CD₁免疫组化染色均呈阳性;3例电镜观察1例示Birbeck颗粒;10例DNA含量流式细胞仪测定6例为异倍体,DNA指数为1.38±0.32,增殖指数为38.69%。检测结果表明:本病皮损中病理组织细胞来源于Langerhans细胞,且本病存在潜在恶变的可能。

关键词: 组织细胞增多症X

Abstract: The skin biopsy specimens from fifteen patients with Langerhans cell histiocytosis were studied. Thirteen out of fifteen cases were positive to S100 protein antibody and peanut agglutinin, but negative to Mac387 and lysozyme antibodies, and three cases were positive for CD₁ antibody by immunohistochemistry. Birbeck granules were demonstrated in one of the three cases by electron microscopy. Ten cases were analysed by flow cytometry, six cases (60%) were aneuploid, DNA index was 1.38±0.32, proliferative index was 38.69%. These results showed that these pathognomonic histiocytes were of Langerhans cell origin and their potential malignancy could not be ruled out.

Key words: Histiocytosis X

王健美, 唐曙, 陈仁贵, 凌波. Langerhans细胞组织细胞增生症的免疫组化电镜及DNA流式细胞计数研究[J]. 中华皮肤科杂志, 1995, 28(4): 222-225.

Wang Jianmei, Tang Shu, Chen Rengui. Immunohistochemical, Ultrastructural Studies and Flow Cytometric DNA Analysis in Langerhans Cell Histiocytosis[J]. Chinese Journal of Dermatology, 1995, 28(4): 222-225.