中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (3): 155-157.

• 论著 • 上一篇    下一篇

聚合酶链反应及转印杂交对尖锐湿疣复发的病因学研究

郭华阳1, 李文维2, 谭骏1, 纪贤文1, 刘昕1, 麦跃2, 赵莉蓉2, 贺萍2, 骆惠军2   

  1. 1. 第三军医大学分子生物学教研室, 重庆630038;
    2. 重庆市大坪医院皮肤科
  • 收稿日期:1994-10-02 修回日期:1995-01-09 出版日期:1995-06-15 发布日期:1995-06-15

Study on the Viral Etiology of Recurring Condyloma Acuminata by Southern Blot Hybridization and PCR

Guo Huayang1, Li Wenwei2, Tan Jun1   

  1. Department of Molecular Biology, Third Military Medical University, Chongqing 630038
  • Received:1994-10-02 Revised:1995-01-09 Online:1995-06-15 Published:1995-06-15

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摘要： 用通用引物介导的聚合酶链反应及其产物斑点杂交技术,对15例复发及相应的原发尖锐湿疣(CA)皮损中人乳头瘤病毒相关序列进行了检测和分型;同时还对35例原发CA周围外观正常皮肤组织DNA作了转印杂交分析。结果表明隐性感染是CA复发的非常重要原因,再感染也是CA复发不容忽视的原因。

关键词: 尖锐湿疣, 聚合酶链反应, 乳头状瘤病毒

Abstract: We studied biopsy specimens taken from normal-appearing skin adjacent to the lesions of 35 cases of condyloma acuminata for HPV related sequences by Southern blot hybridization using digoxigenin-labelled mixed HPV 6b, 11, 16 and 18 DNA probes. The results demonstrated that HPV was detected in 17 out of 35 cases (48.6%). This shows that latent infection of HPV is responsible for the relapse. We also studied 30 biopsy specimens (including 15 original and 15 relapsed lesions taken from 30 cases) and analyzed for HPV sequences by general primer-mediated polymerase chain reaction (PCR) and dot blot hybridization using digoxigenin-labelled specific subtype oligonucleotide probes. The results showed that in 15 relapsed cases the HPV types of 6 cases were different from those of original ones,and 4 of them were.of new types. The data support new infections are responsible for the recurrence.

Key words: Condyloma acuminata, Polymerase chain reaction, Papillomavirus