中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (3): 148-149.

• 论著 • 上一篇    下一篇

直接原位聚合酶链反应检测尖锐湿疣中的人乳头瘤病毒

李惠1, 朱学骏2   

  1. 1. 重庆医科大学附属第一医院皮肤科, 630042;
    2. 北京医科大学第一临床医院皮肤科
  • 收稿日期:1994-05-18 修回日期:1994-09-10 出版日期:1995-06-15 发布日期:1995-06-15
  • 基金资助:
    中华医学基金会资助项目

Detection of HPV DNA in Condylomata Acuminata by Direct in Situ PCR

Li Hui1, Zhu Xuejun 2   

  1. Department of Dermatology, First Affiliated Hospital, Chongqing Medical University, Chongqing 630042
  • Received:1994-05-18 Revised:1994-09-10 Online:1995-06-15 Published:1995-06-15

PDF (PC)

527

赞

摘要： 评价用直接原位聚合酶链反应(PCR)技术辅助诊断尖锐湿疣(CA)的价值及观察人乳头瘤病毒(HPV)在CA中的组织学分布模式。25例CA中,10例具典型组织学特征的CA均有HPV分布,主要位于表皮浅层,另11例缺乏典型空泡细胞的CA在颗粒层或棘细胞全层测得HPV.直接原位PCR通过标记生物素的寡核苷酸在扩增时直接掺入结合,再用免疫组织化学测定,能在石蜡切片上将病毒检测结果与组织学变化直接相比,有助于病理学家对可疑损害作HPV感染的分析。

关键词: 尖锐湿疣, 聚合酶链反应, 乳头状瘤病毒

Abstract: The purpose of this study is to ascertain the significance of diagnosis of human papillomavirus (HPV) infection and the histological pattern of distriution of HPV in condylomata acuminata(CA) by direct in situ PCR (ISPCR) technique. of the 25 patients with BA, all 10 cases with typical histopathological features of CA gave a positive reaction in tile upper layers of the epithelium. HPV was also detected in the granular ladyer and whole prickle cell layer of 11 cases of CA with no typical koilocytes. In the direct ISPCR oligonucleotides labelled with bio tin were incorporated in situ during amplification, then immunohistochemical method was used for detection. In this technique the viral existence in paraffin sections can be directly compared with the histologic changes, and can help pathologist to analyze the possibility that HPV is present in the suspected lesions.

Key words: Condylomata acuminata, Polymerase chain reaction, Papillomaviruses