中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (2): 86-88.

• 论著 • 上一篇    下一篇

增殖细胞核抗原在皮肤鳞癌中的表达

王建力1, 陈莉2   

  1. 1. 南通医学院附属医院皮肤科, 江苏, 226001;
    2. 南通医学院附属医院病理解剖教研室, 江苏, 226001
  • 收稿日期:1994-03-07 修回日期:1994-10-08 出版日期:1995-04-15 发布日期:1995-04-15

Expression of Proliferating Cell Nuclear Antigen in Squamous Cell Carcinoma of the Skin

Wang Jianli1, Chen Li2   

  1. Department of Dermatology, Affiliated Hospital, Nantong Medical College, Nantong 226001
  • Received:1994-03-07 Revised:1994-10-08 Online:1995-04-15 Published:1995-04-15

摘要: 免疫组织化学ABC法检测50例鳞癌细胞核中增殖细胞核抗原(PCNA)的表达.在原位观察肿瘤细胞的增殖活性,探讨其与肿瘤分级、转移的关系.结果显示:PCNA阳性反应定位于细胞核内,在正常上皮中PCNA阳性细胞呈有规律分布;在肿瘤细胞中PCNA阳性呈异质性分布,提示癌细胞生长失调与DNA合成紊乱,同时也反映了癌细胞群体的增殖水平.鳞癌细胞核内PCNA表达与癌细胞分化和转移呈正相关(P<0.05).在鳞癌间质中部分间质细胞与免疫反应细胞也呈PCNA阳性,提示癌细胞通过自分泌和旁分泌生长因子,可同时引起肿瘤本身和周围细胞的增殖.

关键词: 癌,鳞状细胞, 抗原,增殖细胞核

Abstract: The expression of proliferating cell nuclear antigen (PCNA) was studied in 50 cases of squamous cell carcinoma of the skin using immunohistochemistry and PCNA cyclin monoclonal antibody.PCNA staining was found in the nuclei of tumour cells, two types of staining were seen, a granular pattern and a more diffuse nuclear staining.The expression of PCNA showed a significant positive correlation with degrees of differentiation of the carcinoma cells and lymph node metastasis (P<0.05).In some cases the increased expression of PCNA in the tissue adjacent to the tumour may represent the autocrine and paracrine of growth factors of cancer cells which influence the expression of PCNA.

Key words: Carcinoma,squamous cell, Antigen, proliferating cell nuclear