中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (6): 345-346,398.

• 论著 • 上一篇    下一篇

ABC法检测孢子丝菌病病理组织中病原体的研究

张亚芹1, 张民夫2, 段正芳2   

  1. 1. 白求恩医科大学第二临床学院皮肤科, 长春, 130041;
    2. 指导者
  • 收稿日期:1993-11-05 修回日期:1994-04-08 发布日期:1994-12-15

Detection of Organisms in Pathologic Tissues of Sporotrichosis with ABC Technique

ZHANG Ya-Qin1, ZHANG Min-Fu2, DUAN Zheng-Fang2   

  1. The Second Clinical College of N. Bethune University of Medical Sciences, Changchun 130041
  • Received:1993-11-05 Revised:1994-04-08 Published:1994-12-15

摘要: 用ABC-ELISA方法对皮肤抱子丝菌病病理组织中病原体的检测进行了研究.孢子被染成棕褐色,呈圆形、梨形,可见发芽孢子及星状体、巨噬细胞内及白细胞内被吞噬的孢子.孢子直径为2.5um~10.0um.本方法灵敏度为96.2%,特异度为100%.检查1例需要3小时.故认为本方法可用于快速诊断孢子丝菌病及本病的回顾性研究.

关键词: 孢子丝菌病

Abstract: The organisms in pathologic tissues of sporotrichosis were detected by ABC technique.Brown spores were found, some spores appeared to be round, or pearshaped,budding spores or asteriod hotlies were observed, and some spores were phagocytlzed by leukocytes and macrophages.The diameter of spores ranged from 2.spin to 10.0um.The sensitivity of this method is 96.2% and the specificity 100%.Examining one case takes about three hours.The method can be used for retrospective study, as well as rapid diagnosis of sporotrichosis.

Key words: Sporotrichosis