中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (5): 278-280,329.

• 论著 • 上一篇    下一篇

肿瘤坏死因子和γ干扰素对调节中性粒细胞抗白念珠菌活性的研究

江文1, 陈映玲2, 周礼义2   

  1. 1. 同济医科大学附属同济医院皮肤科, 武汉430030;
    2. 指导者
  • 收稿日期:1993-10-16 修回日期:1994-03-29 出版日期:1994-10-15 发布日期:1994-10-15

Regulations of Human Tumor Necrosis Factor and Recombinant Human Interferon-γ on activity of Human Polymorphonuclear Neutrophils against Candida albicans

JIANG Wen1, CHEN Yin-Ling2, ZHOU Li-Yi2   

  1. Department of Dermatology, Tongji Hospital, Tongji Medical University, Wuhan 430030
  • Received:1993-10-16 Revised:1994-03-29 Online:1994-10-15 Published:1994-10-15

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摘要： 通过检测白念珠菌菌落形成单位的方法,观察人类肿瘤坏死因子(Hu-TNF)和重组人类γ干扰素(rHu-IFN-γγ)对人嗜中性粒细胞(PMN)抗白念珠菌活性的调节。发现Hu-TNF和rHu-IFN-γγ本身不能直接影响白念珠菌生长,但能增强人PMN抗白念珠菌生长的活性,这种作用的强弱与其预培养时间有关;此外这两种细胞因子具有协同增强PMN抗白念珠菌活性的作用。这对于增强免疫损伤患者抵抗条件致病真菌感染的能力具有一定的指导意义。

关键词: 肿瘤坏死因子, 干扰素类Ⅱ型, 嗜中性粒细胞, 白念珠菌

Abstract: This paper studies tile regulation of cytokines such as human tumor necrosis(Hu-TNF) and recombinant human interferon-γ(rHu-IFN-γγ) on activity of human polymorphonuclear neutrophils (PMN) against Candida albicans by determing numbers of colony-forming unit of Candida albicans. We detected that Hu-TNF and rHu IFN-γγ did not interfere directly with fungal growth. These two cytokines enhanced PMN to inhibit Candida albicans growth. When preincubating PMN with Hu-TNF and rHu-IFN-γγ togather, the activity of PMN against Candidaalbicans was synergically enhanced. The degree of enhancement of the activity of PMN againstCandida albicans was highly dependent on these two cytokines-PMN preincubation time. Thus,Hu-TNF and rHu-IFN-γγ have the ability to activate PMN, and the synergistic action of thetwo cytokines may prove clinically effective for increasing the ability of immuncompromised hosts against opportunistic fungal infectons.

Key words: Tumor necrosis factor, Interferon type Ⅱ, Neutrophils, Candida albicans