中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (4): 227-228,270.

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小鼠实验性孢子丝菌感染皮损中病原体的形态

刘晓明1, 林熙然2, 孙亦新2   

  1. 1. 大连医科大学附属一院皮肤科, 辽宁, 116011;
    2. 指导者
  • 收稿日期:1993-07-24 修回日期:1993-11-16 出版日期:1994-08-15 发布日期:1994-08-15

A Morphological Study of the Organism in Tissue Sections of Experimental Murine Cutaneous Infection with Sporothrix Schenckii

LIU Xiao-Ming1, LIN Xi-Ran2, SUN YI-Xin2   

  1. Department of Dermatology, The First Affiliated Hospital, Dalian Medical University, Dalian 116011
  • Received:1993-07-24 Revised:1993-11-16 Online:1994-08-15 Published:1994-08-15

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摘要： 接种孢子丝菌于小鼠皮内造成实验性皮损,用多种方法研究病理组织内菌的形态学特点及变化.确认HE染色标本中孢子周围有一环状不着色晕.组织内该菌的凝集素结合形式与其培养相同而有别于多种其他深部真菌.发现半薄切片能更清晰地显示菌体形态及其与宿主细胞的关系.在宿主细胞免疫低下时菌丝可存活并生长繁殖.

关键词: 孢子丝菌属, 小鼠, 病理学

Abstract: Experimental lesions were produced in mice by inoculating Sporothrix schenckii suspension intracuta neously.The morphologic features of the organisms in tissue sections were observed with various methods and studied concerning their significance in histopathological diagnosis.The results defined that with H.E staining,the spores were found to be charac teristically surrounded by a narrow clear halo.The lectin binding pattern of the organisms in tissue sec tions was similar to that in culture,which was different from several other kinds of deep fungi.It was found that the semi-thin section embedded in Epon had an outstanding advantage both in detecting the organisms and showing the relation between the organisms and the host cell.The results showed that the morphologic changes of the organisms in tissue related to the host resistance.

Key words: Sporotrichum, Mice, Pathology