免疫金银染色在大疱病研究中的意义

中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (4): 208-210,268.

免疫金银染色在大疱病研究中的意义

周曙霞¹, 翁孟武², 施守义²

1. 上海医科大学皮肤病学研究所, 200040;
2. 指导者

收稿日期:1993-09-06 修回日期:1993-11-29 出版日期:1994-08-15 发布日期:1994-08-15

The Value of IGSS in Studying Bullous Skin Diseases

ZHOU Shu-Xia¹, WENG Meng-Wu², SHI Shou-Yi²

Institute of Dermatology, Shanghai Medical University, Shanghai 200040

Received:1993-09-06 Revised:1993-11-29 Online:1994-08-15 Published:1994-08-15

摘要/Abstract

摘要： 三种免疫金银染色法(IGSS)──接法、间接法及葡萄球菌A蛋白金标(PAG)法检测自身免疫性大疱病皮损组织32份,先天性大疱性表皮松解症2例和5例正常人皮肤组织作对照,并与直接免疫荧光(DIF)检测结果相比较.同时用包埋后免疫金染色(IGS)技术对疱疹样天疱疮和获得性大疱性表皮松解症各1例作免疫电镜研究.结果表明IGSS不仅在光镜水平检测大疱病皮肤组织中自身抗体优于DIF,而且可从超微结构上了解免疫复合物沉积的确切部位,对自身免疫性皮肤病的诊断和鉴别诊断具有一定价值.

关键词: 免疫金银染色, 皮肤病,大疱性, 显微镜检查,免疫电子

Abstract: We used three kinds of immunogold silver staining (IGSS) direct,indirect and Staphylococcus protein A gold (PAG) to investigate the skin lesions 32 cases with various autoimmune bullous diseases, 2 cages with epidermolysis bullous simplex and 5 normal adults as control, and compared with those by DIF.Postembedding immunogold labelling direct IEM was performed on 1 case with pemphigus herpetiformis and 1 case with epidermolysis bullous acquisita.The results demonstrated that IGSS is not only superior to DIF in investigating the autoantibody of the skin lesions by light microscopy but it is able to be used in IEM in autoimmune bullous diseases.We suggest that IGSS have great value in diagnosis and differential diagnosis of autoimmune bullous diseases.

Key words: Immunogold silver staining, Skin diseases,bullous, Microscopy,immunoelectron

周曙霞, 翁孟武, 施守义. 免疫金银染色在大疱病研究中的意义[J]. 中华皮肤科杂志, 1994, 27(4): 208-210,268.

ZHOU Shu-Xia, WENG Meng-Wu, SHI Shou-Yi. The Value of IGSS in Studying Bullous Skin Diseases[J]. Chinese Journal of Dermatology, 1994, 27(4): 208-210,268.