中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (1): 3-6,61.

• 论著 •    下一篇

表皮郎格罕细胞表达高亲和力IgE-Fc受体

王秉鹤1, Rieger A2, Stingl G2, Ochiai K3, Kinet JP3   

  1. 1. 南京医科大学第一附属医院皮肤科, 210029;
    2. 奥地利维也纳大学医学院皮肤科;
    3. 美国国立卫生研究院(NIH)
  • 收稿日期:1992-10-19 修回日期:1993-03-23 出版日期:1994-02-15 发布日期:1994-02-15

Epidermal Langerhans Cells Express High Affinity IgE-Fc Receptor

Wang Bing-He1, Rieger A. Stingl G2   

  1. Department of Dermatology, Ist Affliatcd Hospilal, Nanjing Medical College, Nanjing 210029
  • Received:1992-10-19 Revised:1993-03-23 Online:1994-02-15 Published:1994-02-15

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摘要： 为了进一步证实抗FcεRImAb特异性地结合至郎格罕细胞(LC)表面,我们用免疫金标记技术进行了免疫电镜检查。发现5nm金颗粒不连续性地分布于LC,而非其它表皮细胞表面,从而证明LC是抗FcεRI反应性表皮细胞。最后,为了了解LC的FcεRI基因表达与否,进行了PCR试验。结果显示在富集LC而非去除LC的表皮细胞悬液中存在FcεRIa、β和γ链的转录物。总之,本研究提供了直接证据证明正常人表皮LC表达FcεRI.

关键词: 郎格罕细胞, 受体,IgE-Fc

Abstract: In order to confirm the specific binding of anti-Fos RI InAb to the surface of LC, IEM using immunogold labeling was performed. We found that LC were anti-Fos RI CAb reactive epidermal cells as evidenced by the discontinuous distribution of sum gold particles along the surface of LC, but no other epidermal cells. Furthermore, in an attempt to search for Fos RI gene expression by LC, PCR experiment was performed and the result revealed the presence of Fos RI al 0 and Y chain transcripts in LC-enriched, but not in LC-depleted, cpidermal cell suspensions. Collectively the present studies Provide direct evidence for the presence of Fos RI on epidcrmal LC.

Key words: Langerhans cells, Receptor,IgE-Fc