中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (1): 10-12,61.

• 论著 • 上一篇    下一篇

氮芥和地塞米松对人角朊细胞血小板活化因子的抑制作用

刘宝军1, 郑茂荣2, 李少华3, 张素珍4, 戴方平4   

  1. 1. 沈阳军区总医院皮肤科, 沈阳110015;
    2. 第二军医大学长海医院皮肤科;
    3. 第二军医大学病理生理教研室;
    4. 第二军医大学烧伤研究所
  • 收稿日期:1992-12-05 修回日期:1993-04-08 出版日期:1994-02-15 发布日期:1994-02-15

Inhibitory Effect of Nitrogen mustard and dexamethasoue on Platelet Activated Factor Synthesis to Cultured human Keratinocytes

LIU Bao-Jun1, Zheng Mao-Rong2, LI Shao-Hua3   

  1. Shenyang Military General Hospital, Shenpeng 110015
  • Received:1992-12-05 Revised:1993-04-08 Online:1994-02-15 Published:1994-02-15

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摘要： 卡西霉素刺激原代培养人角朊细胞合成并释放血小板活化因子(PAF),用洗涤兔血小板聚集法测定。结果表明,氮芥、地塞米松在10-9mol/L～10-5mol/L浓度范围内均能明显抑制角朊细胞PAF生成,并与药物呈剂量和时间依赖关系,氮芥IC50为6.34×10-9mol/L、地塞米松IC50为1.005×10-8mol/L.提示,氮芥和糖皮质激素抑制角遥远朊细胞PAF生成很可能是其治疗银屑病机理的一部分。

关键词: 氮芥, 地塞米松, 血小板激活因子, 角朊细胞

Abstract: We used the method of aggregation of washed rabbit platelet to study the effect of nitrogen mustard and dexamethasone on the synthesis of PAF from calcimycin (A23187) sitmulated human primary cultured keratinocytes and found that both nitrogen mustard and dexamcthasone could does- and timedependently inhibit PAF synthesis. The ICSO of nitrogen mustard and dexamethasone are 6.34×10-9 mol/L and 1.005×10-8 mol/L respectively. The results indicate that the inhibition of PAF synthesis might be part of tile mechanism of action of nitrogen mustard and dexamethasone therapy in psoriasis.

Key words: Mechlorethamine, Dexamethasone, Platelet activating, factor Keratinocyte