中华皮肤科杂志 ›› 1993, Vol. 26 ›› Issue (4): 204-206.

• 论著 • 上一篇    下一篇

聚合酶链式反应快速检测白念珠菌

陈江汉1, 廖万清1, 朱丽华2, 叶增灿2   

  1. 1. 上海长征医院, 200003;
    2. 中国科学院细胞生物学研究所
  • 收稿日期:1992-05-23 修回日期:1992-11-18 出版日期:1993-08-15 发布日期:1993-08-15

Use of polymerase chain reaction for detection of candida aibicans

CHEN Jiang-Han1, LIAO Qing1   

  1. Shanghai Chang Zheng Hospital, Shanghai 200003
  • Received:1992-05-23 Revised:1992-11-18 Online:1993-08-15 Published:1993-08-15

PDF (PC)

211

赞

摘要： 我们应用聚合酶链式反应(PCR)技术休外扩增编码白念珠菌细胞色素P450L1A1的基因片段,在7小时内可以检测出500μ1中含有30个醉母菌的临床标本.对22份临床标本(其中5份明性,17份阳性)同时进行的PCR检测和真菌学鉴定显示:两者具有较满意的一致结果.这17份阳性标本包括4份血标本,5份尿标本,6份痰标本及2份分泌物标本.另5份为阴性血标本.PCR技术在白念珠菌感染乃至其它真菌感染的快速诊断方面显示了良好的应用前景.

关键词: 白念珠菌, 聚合酶链式反应

Abstract: Candida albicans is an opportunitsic pathogenic fungus causing local and systemic disease, the mortality has been increasing dramatically. Early detection and management of candidiasis are crueaal, but conventional methods of diagnosis need long time and have low positivity. The authors used the polymerase chain reaction (PCR) to amplify a fragment of fungal DNA coding for the cytochroma P450 L1AI of C.albirans in vitro. The technique provides unambiguous evidence of C. albicans in 7 hours with, a detection as low as 30 organisms. 22 clinical specimens were callectsd from 15 patients, in each case the result of PCR was confirmed by mycological method. PCR based detection of pathogenic organisms may have broad application in rapid screening for the presence of organisms, and provides rapid and sensitivt methnd for diagnosis.

Key words: Candida albicans, Polymerase chain, reaction