中华皮肤科杂志 ›› 1992, Vol. 25 ›› Issue (4): 219-221.

• 论著 •    下一篇

念珠菌DNA的酶切分析

李春阳1, 吴绍熙1, 郭宁如1, 李银太2, 林万明2   

  1. 1. 中国医学科学院皮肤病研究所;
    2. 军事医学科学院微生物流行病研究所
  • 收稿日期:1991-05-09 修回日期:1991-08-10 出版日期:1992-08-15 发布日期:1992-08-15

Analysis of Candida chromosome DNA by restriction endonuclease

LI Chun-Yang1   

  1. Institute of Dermatology, Chinese Acadsmy of Medical Sciences, Nanjing, 210042
  • Received:1991-05-09 Revised:1991-08-10 Online:1992-08-15 Published:1992-08-15

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摘要： 对6种常见致病性念珠菌DNA进行大肠杆菌甲基化酶(RcoRI)酶切,经琼脂糖凝胶电泳,结果各种念珠菌的酶切带型不同.其中白念珠菌均出现三条EB染色强烈的带,长度为4.9kb,3.7-3.8kb,2.4-2.5kb.试验表明,这三条带型具有较好的稳定性和重复性,提示此为白念珠菌基因的EcoR酶切特点,经进一步证实后,可作为菌种鉴定的一种参考方法.

关键词: 念珠菌属, 脱氧核糖核酸,真菌

Abstract: DNA from six common pathogenic randida species were digested by the restriction endonuclease EcoRI. The geleleckrophoresis revealed different patterns fnr each of the species. Moreaver, eight strains of C. albicans demonstrated three intensely staining bands(4.9, 3.7 to 3.8, 2.4 to 2.5 kilobases in length) and the patterns were shown to be stable and reproducible. It is suggested that the patterns represent genotypic characteristics of C. albicans and after studying for more strains, the DNA restricted patterns may offer a useful tool for the identification of candida species.

Key words: Candida, DNA, fungal