中华皮肤科杂志 ›› 1992, Vol. 25 ›› Issue (3): 162-164.

• 论著 • 上一篇    下一篇

人毛囊乳头细胞的培养

胡俊才1, 韩风霞1, 郭定九2, 张其亮2, 钟值难2   

  1. 1. 湖南医科大学生物教研室 410078;
    2. 湖南医科大学附属第二医院皮肤科
  • 收稿日期:1991-01-18 修回日期:1991-09-26 出版日期:1992-06-15 发布日期:1992-06-15

Cultivation of dermal papilla cells from human hair follicles

HU Jun-Cai1, HAN Feng-Xia1   

  1. Hunan Medical University, Changsha, 410078
  • Received:1991-01-18 Revised:1991-09-26 Online:1992-06-15 Published:1992-06-15

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摘要： 报告了人毛囊乳头细胞培养技术,并对毛囊乳头细胞在体外的生长特点、形态学特点作了动态观察.并采用原位固定的方法进行了扫描电镜观察,用组织化学技术进行了特殊染色,证明人毛囊乳头细胞是一类有别于普通成纤维细胞的、具有特殊形态和生长规律的成纤维细胞亚型.该项细胞培养技术简单可靠,适合于国内普通皮肤科实验室.

关键词: 纤维母细胞, 细胞学

Abstract: A technique of culturing the dermal papilla cells from human hair follicles in vitro is reported. The growth feature and morphology of the cultured cells were studied,and a comparison with fibroblast from root sheath was made contemporaneously. Scanning EM observation and histochemical techniques were used for the study of the cultured cells. The results show that human dermal papilla cells are a specific subpopulation of fibrotrlast in marphaiogy and furtction, and also demonstrate that the culture method used in tills study is sample,reLiable and can be used in the common dermatolosie laboratories.

Key words: Fibroblasts