中华皮肤科杂志 ›› 1992, Vol. 25 ›› Issue (3): 149-151.

• 论著 • 上一篇    下一篇

酶联免疫吸附试验检测抗Ro/SSA抗体

张建中, 韩钢文, 马圣清, 秦俭, 王光超   

  1. 北京医科大学第一医院皮肤科 100034
  • 收稿日期:1991-03-16 修回日期:1991-07-15 出版日期:1992-06-15 发布日期:1992-06-15

Quantitative determination of anti-Ro/SSA antibody by enzyme-linked immunosorbent assay

ZHANG Jian-Zhang, HAN Gang-Wen   

  1. The First Hospital, Beijing Medical University, 100034
  • Received:1991-03-16 Revised:1991-07-15 Online:1992-06-15 Published:1992-06-15

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摘要： 用免疫亲和层析法纯化猪脾Ra/SSA杭原,建立起酶联免疫吸附试验(ELISA)检测抗Ro/SSA抗体的方法.纯化的Ro/SSA在SDS-PAGE分析中为一条60 000蛋白多肤.以100μg/ml浓度包被抗原,1:50稀释血清,测得40例正常人平均OD值为0.10,正常值上限为0.19,83例SLE病人中,69例(83.1%)OD值大于0.19,平均为0.41,显著高于正常人(P<0.05),校正血清筛选使之随机化后,SLE病人抗Ro/SSA抗体阳性率为74.6%.同组病人中,同时用ELISA和CIE检测抗Ro/SSA抗体,结果ELISA法阳性率明显高于CIE法阳性率(P<0.05).我们还就ELISA方法中的一些影响因素进行了讨论.

关键词: 酶联免疫吸附试验, 抗Ko/SSA抗体

Abstract: Antibody to Ra/SSA occurs in patients with syscemic lupus eryihematogus and Sj×gren's syndrome. Ro/SSA antigen was purified by imunoaffinity method with the pig spleen as the antigen source. Purifled Ro/SSA antigen is a peptide of approximatoty 60 000 in SDS-PAGP analysis. By using this purified antigen,a sensitive cnzyme-lirsked immunpsorbent assay(ELTSA) for determination of anti-Ro/SSA antibody was established. The antigen concentration way 100μg per ml and the sera were dilnted to 1/50.

Key words: ELISA, Anti-Ro/SSA antibody