中华皮肤科杂志 ›› 1992, Vol. 25 ›› Issue (2): 75-76.

• 论著 •    下一篇

原位杂交法检测尖锐湿疣患者皮损中人乳头瘤病毒DNA类型

李志刚1, 张玉麟1, 廖万清1, 邵经政1, 陆德如2   

  1. 1. 第二军医大学长征医院皮肤科, 上海, 204403;
    2. 第二军医大学基因工程教研室
  • 收稿日期:1990-11-28 修回日期:1991-06-17 出版日期:1992-04-15 发布日期:1992-04-15

HPV typing in condploma acuminata by in situ hybridization

LI Zhi-Gang1, ZHANG Yu-Lin1   

  1. The Second Affiliated Hospital, Second Military Medical University, Shanghai, 200003
  • Received:1990-11-28 Revised:1991-06-17 Online:1992-04-15 Published:1992-04-15

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摘要： 采用生物素标记的人乳头瘤病毒(HPV)6、11型cDNA探针和尖锐湿疣的石蜡切片做原位杂交检测了B例患者皮损的HPV DNA类型.结果显示2例HPV 6型阳性,其中1例于洽愈后半年内复发合并癌变,提示HPV的癌基因性质;1例2个月后复发,其棘细胞深层及基底细胞层有大片阳性显色,提示可能为复发的病因机制.另外3例HPY11型阳性,1例两型均阴性.

关键词: 尖锐湿疣, 乳头状瘤病毒, 杂交

Abstract: With biotin-labelled HPV (human papilla-mavirus)-6,11 cDNA probes,HPV DNA types were detected in paraffin-ambedded sections of 6 cases of candyloma acumfnata. The result showed that 2 cases were positive for HPV-6, 3 eases positive far HPV-11,and 1 case was negative for both types.In one of the HPV-6 positive cases,recurreace and malignant change of the skin lesion occutred 6 months after clinical cure which may raveal the oncoganic characteristics of HPV,another case relapsed. 2 months later, and large patches of positive staining were observed in the lower squamous cell layer and basal cell layer which may implicate the etiologic mechanism of rccurranca of candylortia acuminata.

Key words: Condylomata acuminata, Pa pillomaviruses, Hybridization