中华皮肤科杂志 ›› 1991, Vol. 24 ›› Issue (6): 375-377.

• 论著 • 上一篇    下一篇

应用抗麻风杆菌单克隆抗体免疫组化技术对麻风诊断新方法的研究

是元甫, 刘季和, 桑红桂, 曾学思, 李杰, 王晓红, 李文忠, 舒会文, 叶干运   

  1. 中国医学科学院皮肤病研究所, 南京 210042
  • 收稿日期:1990-07-29 修回日期:1991-01-15 出版日期:1991-12-15 发布日期:1991-12-15
  • 基金资助:
    国家自然科学基金资助题,中国医学科学院资助题

Study on New Diagnostic Method for Leprosy Using Monoclonal Antibodies Against Mycobacterium Leprae Immunohistochemical Technique

SHI Yuan-Fu, LIU Ji-He   

  1. Institute of Dermatology, Chinese Academy Madical Sciences, Nanjing
  • Received:1990-07-29 Revised:1991-01-15 Online:1991-12-15 Published:1991-12-15

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摘要： 应用5种由荷兰Kolk教授等提供的抗麻风杆菌单克隆抗体,采用免疫组化方法对25例各型麻风(其中LL3例,BL9例、BB1例、BT9例、TT3例)皮损切片进行检测.结果以抗麻风杆菌酚糖明脂单克隆抗体检测抗原结果最好.在BB,BL、及LL病例切片中,其阳性细胞数与切片内抗酸染色阳性程度基本平行一致.而在9例BT中,6例查抗酸杆菌结果为阴性,但用单克隆抗体检查麻风杆菌酚糖脂抗原则为阳性.3例TT查抗酸杆菌阴性,而其中有1例麻风杆菌酚糖脂抗原阳性.此站果表明应用抗麻风杆菌酚糖脂单抗检测组织切片中的抗原,在BT及TT中比抗酸染色方法较为优越.

Abstract: Using 5 kinds of anti-M leprae monoclonal antibodies(kindly proided by Prof.Arend HJ Kole etc.form the Netherlands) and immuoohistochemical technique,the lesioual skin sections of 25 cases of leprosy(including 3 cases of LL,9 BL,1 BB,9 BT and 3 TT) were examined.Tlte results showed that antiphenolic glycolipid(PGI)McAb was the best one in the five in demonstrating bacterial antigen.In the lesions of BB,BL,and LL patients,the number of PGI-positive cells were basically parallel to and consistent with the positirrity of acidfast stain.But in 6 of 9 Cases of CT,acid-fast stain was negative,while PGI was positive in all 9 cases.In thesections of 3 cases of TT with acid-fast negative,one reveaied PGI positive The results indicated that in BT and TT,the detection of PGI antigen using anti-PGI McAb technique was superior to that of routine method with acidfast stain.