中华皮肤科杂志 ›› 1991, Vol. 24 ›› Issue (5): 323-325.

• 论著 • 上一篇    下一篇

致病隐球菌DNA中鸟嘌呤加胞嘧啶摩尔百分比含量的研究

赵志强, 廖万清   

  1. 中国人民解放军第二军医大学长征医院皮肤科
  • 收稿日期:1990-03-12 修回日期:1990-06-15 出版日期:1991-10-15 发布日期:1991-10-15

Determination of Guanine and Cytosine Contents of DNA in Some Pathogenic Cryptococci

ZHAO Zhi-Qiang, LIAO Wan-Qing   

  1. Chang Zheng Hoapital, Shanghai
  • Received:1990-03-12 Revised:1990-06-15 Online:1991-10-15 Published:1991-10-15

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摘要： 用热变性法测定了致病隐球菌DNA核普酸组成中的硷基比率.结果罗伦隐球菌最低,新型隐球菌最高,新型隐球菌上海变种介于罗伦隐球菌和新型隐球菌格特变种之间.其变异范围为47.82-61.98.用蜗牛酶辅以十二烷基磺酸钠(SLS)破壁,克服了隐球菌壁厚且有夹膜难以提取DNA的问题.

Abstract: The G+C mol% content of some species of Cryptococei was reported here as determined by a thermal denaturation iechninue.The results showed that the G+C mol% content was lowest in C.lurentii,highest in C.neoformans,and Shanghai variant between C.laurentii and C.gatlii variant the range of them was from 47.82 to 61.98%,which are similar to those reported in the literatures.