中华皮肤科杂志 ›› 1991, Vol. 24 ›› Issue (5): 291-293.

• 论著 •    下一篇

免疫放射测定法诊断新型隐球菌性脑膜炎

温海, 邵经政, 廖万清, 沈新义, 耿培兰   

  1. 上海长征医院皮肤科 200003
  • 收稿日期:1990-04-10 修回日期:1990-08-09 出版日期:1991-10-15 发布日期:1991-10-15

The Diagnosis of Cryptococcal Meningitis with Immunoradiometric Assays (IRMA)

WEN Hai, SHAO Jing-Zheng   

  1. Shanghai Chang Zheng Hospital
  • Received:1990-04-10 Revised:1990-08-09 Online:1991-10-15 Published:1991-10-15

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摘要： 测定了65份正常脑脊液标本和16份新型隐球菌性脑膜炎病人的脑脊液.免疫放射测定的敏感度为2.75ng/ml.定性分析是根据检测标本和阴性对照标本的比值,超过或等于2.1即为阳性.低于2.1为阴性,结果16份病人标本全部在2.11.以上,最高达6.5,最低为2.9,平均为4.1,定量分析是用纯化的新型隐球菌荚膜多糖(CCP)抗原制做标准曲线,以65份.正常的脑脊液标本获得的正常值为4.34ng/ml,16份脑膜炎病人脑脊液标本内CCP含量显著升高,最高达1000ng/ml以上,最低为128ng/ml,超出正常值29倍.以上结果证明该方法对新型隐球菌性脑膜炎的诊断是有帮助的.

Abstract: The purpose of this study is to establish a method with which cryptococcal capsular polysaccharide (CCP) in cerebrospinal fluid (CSF) of cryptococcal meningitis patients can be detected by Immunoradiometric Assays (IRMA),then put it into use for the clinical diagnosis of cryptococcal meningitis.