中华皮肤科杂志 ›› 1990, Vol. 23 ›› Issue (5): 311-313.

• 论著 • 上一篇    下一篇

ABC-ELISA法诊断隐球菌脑膜炎

苏飞腾1, 邵经政1, 廖万清1, 孔宪涛2, 沈新义2, 耿培兰2   

  1. 1. 第二军医大学附属长征医院皮肤科;
    2. 第二军医大学附属长征医院实脸科
  • 收稿日期:1989-03-30 修回日期:1989-07-24 发布日期:1990-10-15

Rapid diagnosis of cryptococcal aneningitis with ABC-ELISA

SU Fei-Teng1, SHAO Jin-Zhen1   

  1. The Second Military Medical University, Shanghai
  • Received:1989-03-30 Revised:1989-07-24 Published:1990-10-15

PDF

286

赞

摘要： 本文在真苗病领域首次建立了ABC-ELISA法诊断隐球菌脑膜炎,取得满意结果.用本法检测15例经墨汁涂片及培养确诊的隐球菌脑膜炎的脑脊液标本,阳性OD492/阴性OD992比值大于2.1.以常见的致病真菌及难以与隐球菌脑膜炎鉴别的结核性脑膜炎的致病菌一结核杆菌为抗原,用本法检测,P/N值均小于2.1而表现较高的特异性.本法的组内变异系数及组间变异系数分别为5.985%和6.38%,检侧新型隐球菌荚膜多糖(CCP)的灵敏度可达1.95ng/ml,有高重复性和高灵敏度.因而本法不失为快速诊断隐球菌脑膜炎的新方法.

Abstract: Avidin-Biotin-Peroxidase Clomplex(ABC).ELISA was established to diagnose coccal meningitis successfully.The result showed that it had high specificity, and it could detect cryptoonecal capsular polysae charidc (CCP) at the concentration. of 1.95ng/ml. It was convenient, in practising. The result could be read in 8 hours. It was suggested that ABC-BLISA war a sensitive and specific sew method in the rapid diagnosis of cryptococcal meningitis.