中华皮肤科杂志 ›› 1990, Vol. 23 ›› Issue (3): 161-162.

• 论著 • 上一篇    下一篇

1M氯化钠分离皮肤法测定循环IgA抗基底膜带抗体

苏士雄1, R. P. Hall2   

  1. 1. 广西医学院附属医院皮肤科;
    2. 美国Duke大学医学中心
  • 收稿日期:1988-05-25 修回日期:1989-10-30 出版日期:1990-06-15 发布日期:1990-06-15

1M NaCl (SODIUM CHLORIDE)SPLITSKIN AS A SUBSTRATE FOR THE DETECTION OF CIRCULATING IGA-ANTI-BASEMENT ZONE ANTIBODIES

SU Shi-Xiong1, R. P. HALL2   

  1. 1. Guangxi Medical Gollege, Nanning;
    2. Univcrsity Medical Center, U.S.A.
  • Received:1988-05-25 Revised:1989-10-30 Online:1990-06-15 Published:1990-06-15

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摘要： 本文报道用间接免疫荧光法,分别以1M氯化钠分离的正常人皮肤和完整的正常人皮肤为底物,检查循环IgA抗基底膜带(BMZ)抗体。本文检查了疱疹样皮炎(DH),线状IgA大疱性皮肤病(LABD)及大疱性类天疱疮(BP)共39份血清,结果显示,应用分离的正常人皮肤作底物,比应用完整的正常人皮肤作底物敏感。

Abstract: The sera of 11 patients with linear IgA bullous dermatosia, 21 patients with dermatitis herpetiformis, 7 patients with bullous pemphi-goid and 12 normal subjects were examined for IgA-anti-basement membrane zone (BMZ) antibodies (Ab) using both 1M NaCl split normal human skin (NHS) and untreated NHS as substrate in indirect immunofluore-seance. This data suggests that 1M NaCl split NHS may be a more sensitive substrate than untreated NHS for the detection of IgA-anti-BMZ Ab.