中华皮肤科杂志 ›› 1989, Vol. 22 ›› Issue (5): 299-301.

• 论著 • 上一篇    下一篇

抗角蛋白单克隆抗体免疫组化方法的探讨

王晓红, 是元甫, 桑红桂, 李阿梅, 曾学思, 孔庆英, 刘季和   

  1. 中国医学科学院皮肤病研究所
  • 收稿日期:1988-06-29 修回日期:1988-09-23 出版日期:1989-10-15 发布日期:1989-10-15

Immunohistochemical study of monoclonal anti-keratin antibodies

WANG Xiao-Hong, SHI Yuan-Fu   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing
  • Received:1988-06-29 Revised:1988-09-23 Online:1989-10-15 Published:1989-10-15

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摘要： 本文用抗角蛋白单克隆抗体AE3在43例银屑病和9例正常皮肤标本中对冰冻切片免疫冰冻切片间接免疫荧光法和石蜡切片免疫酶标ABC法进行了比较.结果显示:这3种方法在抗角蛋白单克隆抗体的染色方面结果几无差异.同时,其中20例石蜡标本还在保存9个月以后再次切片染色,结果亦无差异.由于石蜡切片免疫酶标ABC法设备条件要求低,易于在基层推广,标本能长期保存,且切片中组织结构保存良好,因此是一种值得推荐的免疫组化方法.

Abstract: In this paper, the merits and deriierits of Immunolustochemical stain of paraffin section were discussed methodologically in comparison with the immunohistochemical and immunofluorescent stain of frozen section by the investigation on psoriatic and normal skin specimens using monoclonal antikeratin antibody AE3.The result indicates that the immunohisto-chemical stain of paraffin section is as good as frozen section and the indirect immuno-fluorescent stain of frozen seetion. Besides, the paraffin section can be stored longer, and has well preserved histologic structure. There-fore, the immunohistochemical stain of paraffin section is a good method to be recommended.