中华皮肤科杂志 ›› 1988, Vol. 21 ›› Issue (1): 6-7.

• 论著 • 上一篇    下一篇

不同底物检测抗核抗体的研究

陈泽仪, 周同, 李卫平, 张传钧   

  1. 上海第二医科大学瑞金医院
  • 收稿日期:1986-10-03 发布日期:1988-02-15

STUDY OF ANTINUCLEAR ANTIBODY BY IMMUNOFLUORESCENGE WITH DIFFERENT SUBSTRATED

Chen Zeyi, Zhou Tong   

  1. Rui-jin Hospital, Shanghai Second Medical University, Shanghai
  • Received:1986-10-03 Published:1988-02-15

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摘要： 以大鼠器官印片、人癌印片和人鳞状上皮癌细胞(HEp-2)作底物,采用间接免疫荧光法在59例各种结缔组织病患者中研究抗核抗体.人癌印片检测ANA的敏感性和特异性均高于动物器官底物,人癌印片所见的着丝点抗体经HEp-2细胞底物证实.

Abstract: Antinucjear antibodies (ANA) were studied in 59 patients with different connective tissue diseases by indirect immunofluorescence (FF) with rat organ imprints, human cancer imprints and HEp-2 cells as substrates. Sensitivity and specificity of human cancer imprints for detection of ANA were higher than animal organ substrates. The finding of anti-centromer.antibody by imtnunofluorescence (IF) with human cancer imprints as substrate was confirmed by IF technique using HEp-2 cells.