中华皮肤科杂志 ›› 1987, Vol. 20 ›› Issue (6): 324-327.

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酶联免疫吸附试验检测血清抗天然DNA杭体

强能贤, 仇宁, 金婉清, 陈军杰, 钟铭英, 徐文严   

  1. 中国医学科学院皮肤病研究所
  • 收稿日期:1987-01-21 修回日期:1987-03-14 出版日期:1987-12-15 发布日期:1987-12-15

Detertion of serum anti-nDNA antibody using ELISA

Qiang Nengxian   

  • Received:1987-01-21 Revised:1987-03-14 Online:1987-12-15 Published:1987-12-15

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摘要： 本文报道以硫酸鱼精蛋白预先处理聚苯乙烯微量滴定板后,用ELISA检测血清抗nDNA抗体的方法,并对试验中的若千影响因素作了初步研究.结果表明,此方法敏感,重复性好,配制的nDNA贮存液约可应用6个月.用此方法检测,确定以血清1/10稀释,OD≥1.0为阳性标准,则76.7%的SLE和90%的活动性SLE病人血清杭nDNA抗体阳性,并对SLE有较高的特异性.

Abstract: The detection of serum anti-nDNA antibody using ELISA pretreated with protamin sulfate is reported in this paper Preliminary research on a number of influential factors in the assay were also made. The results suggest that this method is sensitive and reproducible, and the nDNAs stock solution can be used for about 6 month. According to the criteria defined as positive at 1/10 serum dilution and OD≥1.0, 76.7% of the patients with SLE and 90% of the active SLE patients are positive.