获得性大疱性表皮松解症的诊断—1M NaCl分离皮肤法

中华皮肤科杂志 ›› 1987, Vol. 20 ›› Issue (4): 201-203.

获得性大疱性表皮松解症的诊断—1M NaCl分离皮肤法

沈丽玉

北京医科大学第一医院皮肤科

收稿日期:1986-06-20 修回日期:1986-09-11 出版日期:1987-08-15 发布日期:1987-08-15

IF diagnosis of epidermolysis bullosa acquisita using 1M NaCl to separate human skin

Shen Liyu

Received:1986-06-20 Revised:1986-09-11 Online:1987-08-15 Published:1987-08-15

摘要/Abstract

摘要： 本文报导用间接免疫荧光技术,以1MNaCl分离正常皮肤为底物,其分离部位在透明板,筛选128份含有抗基底膜抗体IgG的疱病血清,以确定其中有多少为EBA,结果102例血清为表皮型,9例为联合型(表皮和真皮均着色),8例为真皮型,9例为阴性.结果显示诊断为类天疱疮患者中大约有10%为EBA,同时详细介绍了皮肤分离法.

Abstract: In order to determine how often epider-molysis bullosa acquisita might be misdia-gnosed as bullous pemphigoid, we screened 128 samples of ‘bullous pemphigoid' sera with BMZ IgG antibodies. Indirect IgG immunofluoresence was performed on the substrates using 1M NaCl to separate human skin through the lamina lucida. The results showed that 102 sera were epidermal staining pattern, nine were epidermal and dermal pattern, eight were dermal pattern, and nine were negative. Among those diagnosed as bullous pemphigoid about 10% were actually EBA patients. The details of how to split the skin were also given.

沈丽玉. 获得性大疱性表皮松解症的诊断—1M NaCl分离皮肤法[J]. 中华皮肤科杂志, 1987, 20(4): 201-203.

Shen Liyu. IF diagnosis of epidermolysis bullosa acquisita using 1M NaCl to separate human skin[J]. Chinese Journal of Dermatology, 1987, 20(4): 201-203.