绿蝇短膜虫免疫荧光法检测dsDNA抗体与间接血凝法对比研究

中华皮肤科杂志 ›› 1987, Vol. 20 ›› Issue (2): 81-82.

绿蝇短膜虫免疫荧光法检测dsDNA抗体与间接血凝法对比研究

葛以信, 张美华, 毕志刚, 陈希萍

南京医学院第一附属医院皮肤科

收稿日期:1986-04-07 修回日期:1986-06-16 出版日期:1987-04-15 发布日期:1987-04-15

Study of Crithidia lucilia immunofluorescence assay in the detection of anti-dsDNA antibodies in comparason with indirect hemagglutination assay

Ge Yixin

Received:1986-04-07 Revised:1986-06-16 Online:1987-04-15 Published:1987-04-15

摘要/Abstract

摘要： 本文介绍了69例中SLE50例,其他结缔组织病19例,应用绿蝇短膜虫免疫荧光法险测dsDNA抗体,并与间接血凝法进行了对照,同时进行了间接免疫荧光检测抗核抗体.结果发现活动性SLE,绿蝇短膜虫检测阳性率为95%,间接血凝法阳性率为45%,两者具有显著性差异(P<0.01),并强调短膜虫检测抗dsDNA抗体可作为判断SLE活动的指标,亦有助于活动性SLE的鉴别诊断.

Abstract: Anti-dsDNA antibodies of 69 cases(50 SLE and 19 other connective tissue diseased were measured with Crithidia luciliae immunofluorescence assay (CL-IF) and indirect hemag-glutination assay (IHA). ANA of there patients was also detected by indirect immunofluorescence at the same time. Active SLE group showed a higher percentage of positive test with CL-IF (95%) than with IHA (45%), the difference was significant (P<0.01). The author emphasized the importance of CL-IF in the evaluation of SLE activity, and its value in the differential diagnosis of active SLE.

葛以信, 张美华, 毕志刚, 陈希萍. 绿蝇短膜虫免疫荧光法检测dsDNA抗体与间接血凝法对比研究[J]. 中华皮肤科杂志, 1987, 20(2): 81-82.

Ge Yixin. Study of Crithidia lucilia immunofluorescence assay in the detection of anti-dsDNA antibodies in comparason with indirect hemagglutination assay[J]. Chinese Journal of Dermatology, 1987, 20(2): 81-82.