中华皮肤科杂志 ›› 1986, Vol. 19 ›› Issue (4): 191-194.

• 论著 •    下一篇

FANA试验中底物敏感性及特异性的研究——组织培养细胞与鼠肝印片对比

王秉鹤1, 钱齐宏1, 康辉波1, 赵辨2   

  1. 1. 南京医学院一附院皮肤免疫研究室;
    2. 指导者
  • 收稿日期:1985-10-31 修回日期:1985-12-27 出版日期:1986-08-15 发布日期:1986-08-15

Fluorescent antinuclear antibody test using both tisssue culture cells and rat liver as substrates

Wang Binghe1   

  • Received:1985-10-31 Revised:1985-12-27 Online:1986-08-15 Published:1986-08-15

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摘要： 作者同时用组织培养细胞(Wish细胞)和鼠肝作为底物进行荧光抗核抗体(FANA)试验.通过对103例结缔组织病和其他自身免疫病的血清检测,比较了两种底物的敏感性,通过16例非自身免疫病和40例健康人血清检测,比较了两者的特异性.结果显示组织培养细胞作底物能明显提高试验的敏感性,而特异性无明显降低.作者提议我国实验室也应逐步改用组织培养细胞或联合使用组织培养细胞与鼠肝作底物,以提高ANA的检出率.

Abstract: Fluorescent antinuclear antibody (FANA) test using both tissue culture cells (Wish cell) and rat liver as substrates were performed. Sensitivities were compared using sera from 103 patients with connective tissue diesases and other autoimmune diseases. Specificities were compared using sera from 16 patients with nonautoimmune diseases and 40 healthy subjects. Results showed that tissue culture cells as substrate could enhance the sensitivity and did not decrease the specificity significantly. The authors consider that it may be of help to use tissue culture cells or combine it with rat liver as substrate in the detection of ANA.