间接血凝法和对流免疫电泳法测定抗ENA抗体

中华皮肤科杂志 ›› 1986, Vol. 19 ›› Issue (3): 145-147.

间接血凝法和对流免疫电泳法测定抗ENA抗体

陈德利¹, 刘松林¹, 顾建中², 朱敏², 沈宜洁², 陈集舟³, 郭伟³, 杨虎天⁴

1. 上海铁道医学院附院皮肤科;
2. 上海铁道医学院附院检验科;
3. 北京医科大学第一医院皮肤科;
4. 上海市第一人民医院内科

收稿日期:1985-05-27 修回日期:1985-11-08 出版日期:1986-06-15 发布日期:1986-06-15

Identification of antibodies to ENA using passive hemagglutination and counterimmunoelectrophotesis

Chen Deli¹

Received:1985-05-27 Revised:1985-11-08 Online:1986-06-15 Published:1986-06-15

摘要/Abstract

摘要： 本文分析和比较了被动间接血凝法(PHA)和对流免疫电泳(CIE)检测抗可提取性抗原(ENA)抗体的结果.发现SLE患者中35,700(60/68)抗RNP抗体阳性,39.9(67/168)抗Sm抗体阳性.15例MCTD中抗RNP抗体均阳性10000 PHA法检测抗Sm抗体敏感,而CIE法检测抗RNP抗体敏感.在PHA法中56℃加热一小时处理ENA和RNase消化ENA结果相似.

Abstract: The results of the detection and identification of antibodies to extractable nuclear antigens(ENA) by the use of passive hemagglutination(PHA) and counterimmunoelectrophoresis(CIE) test concomitantly are analysed and compared in this article.It was found that 60 of 168 samples of sera (35.7%)from patients with SLE showed antibody to ribonucleopr tein(RNP) component of ENA, and 67(39.9%) had antibody to the Sm antigen. All sera from 15 patients with MCTD(100%) had antibody to the RNP.PHA was more sensitive for detection of anti-Sm,while CIE detected more anti-RNP.Heating of ENA at 56℃ for 1 hour has the same effect as the use of ribonuclease in the differentiation of RNP and Sm antibodies in PHA test.

陈德利, 刘松林, 顾建中, 朱敏, 沈宜洁, 陈集舟, 郭伟, 杨虎天. 间接血凝法和对流免疫电泳法测定抗ENA抗体[J]. 中华皮肤科杂志, 1986, 19(3): 145-147.

Chen Deli. Identification of antibodies to ENA using passive hemagglutination and counterimmunoelectrophotesis[J]. Chinese Journal of Dermatology, 1986, 19(3): 145-147.