中华皮肤科杂志 ›› 1986, Vol. 19 ›› Issue (3): 129-133.

• 论著 •    下一篇

酶处理的人精子头核免疫荧光试验及其用于检测AnDNA的研究

陈孟禄1, 陈受2, 李世荫1,3, 费远1   

  1. 1. 北京医科大学第三附属医院皮肤科;
    2. 北京医科大学第三附属医院同位素室;
    3. 指导者
  • 收稿日期:1985-08-31 修回日期:1985-12-02 出版日期:1986-06-15 发布日期:1986-06-15

Study on enzymlysed human spermatozoa nuclei immunofluorescence test and its use in determination of anti-DNA antibody

Chen Menglu1   

  • Received:1985-08-31 Revised:1985-12-02 Online:1986-06-15 Published:1986-06-15

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摘要： 本文试用a-糜、胰蛋白酶处理的人精子头核间接免疫荧光试验检测血清中抗天然脱氧核糖核酸抗体,并与常规方法配对比较.发现:以血清1/10释稀为准,本法的定性结果和标准法基本相符,且其滴度能反映放免法量上的变化,在病种上,本法的敏感性和特异性介于放免法和短膜虫免疫荧光法之间.本法较简便易行,有一定实用意义.

Abstract: Using human spermatozoa nuclei treated with chymotrypsin and trypsin as antigen substrate, the anti-DNA antibody was determined with indirect immunofluorescence test and compared in matched pairs with conventional methods.It was found that with 1/10 dilution of serum as limit, the result of this method(ESN-IFT) corresponded to that of conventional methods.The titer of ESNIFT reflected the quantitative change as determined with RIA.Tlie sensitivity and specificity of this test was just between RIA and CL-IFT.Because the ESN-IFT has the advantage of simplicity and easiness,so it has some practical significance.