麻风患者的T淋巴细胞与酸性非特异醋酶活性的研究

中华皮肤科杂志 ›› 1981, Vol. 14 ›› Issue (3): 139-142.

麻风患者的T淋巴细胞与酸性非特异醋酶活性的研究

强能贤¹, 马林¹, 王东怀¹, 顾生贵¹, 冯壁², 史璋瑛²

1. 中国医学科学院皮肤病研究所;
2. 江苏省泰兴县滨江医院

出版日期:1981-09-15 发布日期:1981-09-15

Investigation of T Lymphocytes and Acid Non-specific Esterase Activity in Leprosy Patients

Qiang Nengxian¹

Online:1981-09-15 Published:1981-09-15

摘要/Abstract

摘要： T和B淋巴细胞的鉴别,可采用花环形成试验、免疫荧光染色、细胞电泳和扫描电镜检查等方法.自1975年Mueller等^①首次报告酸性α-乙酸蔡醋酶(acid a-naphthyl acetate esterase,简称ANAE)活性是小鼠T淋巴细胞的特征以来,用酸性非特异醋酶细胞化学染色法来鉴别人类各种组织内的T和B淋巴细胞的亚群,不仅方法简便易行,而且在一些疾病临床实践上的应用(如白血病的鉴别诊断、肿瘤细胞免疫的观察指标等)已受到人们的重视^(2~8).

Abstract: Using the active E-Rosette test, total E-Rosette test, and acid α-naphthyl acetate esterase(ANAE)cytochemical staining method, the percentage of Ea RFC, Et RFC, ANAE-positive lymphocytes, ANAE-positive Ea RFC and Et RFC in peripheral blood were detected in 76 patients with active leprosy(lepomatous leprosy 46, tuberculoid leprosy 30).The results showed that the percengages of Ea RFC and Et RFC of both groups of leprosy patients were lower than that of the normal individuals.The decrease of ANAE-positive lymphocytes in L leprosy patients was more marked than in T leprosy patients.These findings suggest that active L and T leprosy patients lack mature T lymphocytes and T lymphocytic population with the receptor of sheep erythrocyte in peripheral blood, especially in L patients.Thus, ANAE staining method may be used as a useful approach to determine clinically the state of cell mediated immunity of leprosy patients and to investigate the subpopulation of lymphocytes.

强能贤, 马林, 王东怀, 顾生贵, 冯壁, 史璋瑛. 麻风患者的T淋巴细胞与酸性非特异醋酶活性的研究[J]. 中华皮肤科杂志, 1981, 14(3): 139-142.

Qiang Nengxian. Investigation of T Lymphocytes and Acid Non-specific Esterase Activity in Leprosy Patients[J]. Chinese Journal of Dermatology, 1981, 14(3): 139-142.