中华皮肤科杂志 ›› 1981, Vol. 14 ›› Issue (1): 1-4.

• 论著 •    下一篇

间接血凝法在测定SLE患者血清中抗ds-DNA抗体的意义

康克非1, 施守义1, 侯爱琴1, 邓守贞2, 林祥通2   

  1. 1. 上海第一医学院华山医院皮肤科兔疫室;
    2. 上海第一医学院华山医院同位素室
  • 出版日期:1981-03-15 发布日期:1981-03-15

The Significance of Indirect Hemaggiutination Method in Detecting Serum ds-DNA Antibody in SLE

Kang Kefei1, Shi Shouyi1, Hou Aiqin1, Deng Shouzhen2, Lin Xiangtong2   

  1. 1. Department of Dermatology, Hua Shan Hospital: Shanghai 1st Medical College;
    2. Department of Isotopy, Hua Shan Hospital: Shanghai 1st Medical college
  • Online:1981-03-15 Published:1981-03-15

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摘要： 抗核抗体(ANA)的测定已较普遍地应,用于临床诊断,由于它的非特异性对诊断系统型红斑性狼疮(SLE)仍有一定的局限性.抗双链去氧核糖核酸(ds-DNA,以下简称DNA)抗体的测定对诊断SLE有较大的特异性(1,2),目前已用作ANA侧定后进二步明确诊断、了解病情特别是反映狼疮性肾炎的有用实验室指标之③.测定坑DNA抗体的方法很多,考虑到方法的简便,易于推广,我们采用了间接血凝法.

Abstract: Employing the indirect hemagglutination method and compared with isotopic method, serum anti-ds-DNA antibody was determined in twenty three patients with SLE and nine patients with other connective tissue diseases including mixed connective tissue disease(MCTD), progress systemic scleroderma, Sjogren's syn.drorile and dermatomyositis, Serum ds-DNA antibody has not been found in patients with other connective tissue diseases except one case of MCTD (125I-DNA antibody 25u).The determination of serum anti-ds-DNA, antibody is iinportaut for further analysis of the total anti-nuclear antibody, diagnosis of SLE, understanding the condition of the disease and reflecting nephritis. The result's of indirect hemagglutination and isotopic methods are consistent with each other.The latter is more sensitive,but the former is more available for routine,laboratories.