中华皮肤科杂志 ›› 2019, Vol. 52 ›› Issue (11): 836-839.doi: 10.35541/cjd.20180840

• 研究报道 • 上一篇    下一篇

应用Morris水迷宫实验评估银屑病对小鼠模型认知功能的影响

何磊1    陈军1    陈丽莉1    李雪飞1    王皓2    刘利君1    秦树存2    李保强1    段昕所1   

  1. 1承德医学院附属医院皮肤病与性病科，河北  067000；2山东第一医科大学动脉粥样硬化研究所，山东泰安  271000
  • 收稿日期:2018-10-29 修回日期:2019-05-25 发布日期:2019-11-04
  • 通讯作者: 段昕所 E-mail:duanxinsuo2002@163.com
  • 基金资助:
    承德市科学技术研究与发展计划项目（201606A061）；山东省泰山学者岗位专项科研基金（ts201511057）

Evaluating the impact of psoriasis on cognitive function in mouse models by using Morris water maze

He Lei1, Chen Jun1, Chen Lili1, Li Xuefei1, Wang Hao2, Liu Lijun1, Qin Shucun2, Li Baoqiang1, Duan Xinsuo1   

  1. 1Department of Dermatology and Venereology, Affiliated Hospital of Chengde Medical University, Chengde 067000, Hebei, China; 2Institute of Atherosclerosis, Shandong First Medical University, Taian 271000, Shandong, China 
  • Received:2018-10-29 Revised:2019-05-25 Published:2019-11-04
  • Contact: Duan Xinsuo E-mail:duanxinsuo2002@163.com
  • Supported by:
    Chengde Science and Technology Plan Project （201606A061）; Taishan Scholars Foundation of Shandong Province （ts201511057）

PDF

0

PDF (Mobile)

0

赞

摘要： 【摘要】 目的 通过Morris水迷宫实验评估银屑病对小鼠空间学习和记忆功能的影响。方法 10月龄雄性健康C57BL/6J小鼠20只，根据随机数字表法随机等分为对照组和银屑病组。银屑病组背部外用5%咪喹莫特乳膏，对照组背部外用凡士林，均每日1次，连续1周。建模成功后，应用Morris水迷宫实验评价两组小鼠空间学习和记忆能力。结果 在定位航行实验中，银屑病组逃逸潜伏期［（38.24 ± 13.59） s］明显长于对照组［（14.28 ± 3.80） s］，t = 5.37，P＜0.01。空间探索实验中，银屑病组平台穿越次数（1.70 ± 0.95）明显低于对照组（5.00 ± 1.76），t = 5.21，P＜0.01，平台象限停留时间（t = 2.80，P＜0.05）及平台象限游程（t = 5.74，P＜0.01）均显著低于对照组；银屑病组在第二象限游程较对照组降低（t = 2.49，P＜0.05），但在第四象限停留时间较对照组延长（t = 2.46，P＜0.05），在其他象限停留时间及游程两组间差异均无统计学意义（P＞0.05）。结论 银屑病小鼠模型空间学习和记忆功能发生障碍。

关键词: 银屑病； 认知障碍； 学习障碍； 记忆障碍； 疾病模型, 动物； Morris水迷宫实验

Abstract: 【Abstract】 Objective To evaluate the impact of psoriasis on spatial learning and memory abilities in mouse models by using Morris water maze. Methods Twenty healthy male C57BL/6J mice aged 10 months were randomly and equally divided into 2 groups: psoriasis group topically treated with imiquimod 5% cream on the back once a day for a week, and control group topically treated with vaseline once a day for a week. After successful establishment of mouse models, the Morris water maze （MWM） test was used to assess the learning and memory abilities in the mice in the 2 groups. Results In the place navigation experiment, the escape latency was significantly longer in the psoriasis group （38.24 ± 13.59 s） than in the control group （14.28 ± 3.80 s, t = 5.37, P < 0.01）. In the spatial probe test, the number of times passing through the platform （1.70 ± 0.95 vs. 5.00 ± 1.76, t = 5.21, P < 0.01）, the duration of stay in the target quadrant （t = 2.80, P < 0.05） and the swimming distance （t = 5.74, P < 0.01） were all significantly lower in the psoriasis group than in the control group. The psoriasis group showed significantly decreased swimming distance in the second quadrant （t = 2.49, P < 0.05）, but significantly longer duration of stay in the fourth quadrant compared with the control group （t = 2.46, P < 0.05）. There were no significant differences in swimming distance or duration of stay in other quadrants between the psoriasis group and control group （all P > 0.05）. Conclusion The spatial learning and memory abilities were impaired in the mouse model of psoriasis.

Key words: Psoriasis, Cognition disorders, Learning disorders, Memory disorders, Disease models, animal, Morris water maze