中华皮肤科杂志

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人汗腺腺上皮细胞快速分离培养的初步研究

雷霞, 伍津津, 鲁元刚, 朱堂友   

  1. 第三军医大学大坪医院野战外科研究所皮肤科 重庆 400042
  • 收稿日期:2005-05-26 出版日期:2006-02-15 发布日期:2006-02-15
  • 基金资助:
    国家863课题(2001AA216041;2003AA205020);重庆市重点攻关课题(7897)

Preliminary study on rapid isolation and culture of sweat gland epithelial cells

LEI Xia, WU Jin-jin, LU Yuan-gang, ZHU Tang-you   

  1. Department of Dermatology, Daping Hospital, Third Military Medical University, Chongqing 400042, Sichuan, China
  • Received:2005-05-26 Online:2006-02-15 Published:2006-02-15

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摘要： 目的 体外分离、培养及鉴定人汗腺腺上皮细胞,观察其生长特性.方法 在解剖显微镜下挑取汗腺,用胶原酶消化法将其消化成单个细胞,接种后观察其生长情况,并用上皮膜抗原、角蛋白K19和肌动蛋白进行免疫组化鉴定.结果 细胞生长良好,约3周左右长满瓶底,呈铺路石样生长.免疫组化示抗上皮膜抗原、角蛋白K19阳性,抗肌动蛋白阴性,证明分离培养的细胞为汗腺腺上皮细胞.结论 建立了一种体外快速分离和培养人汗腺腺上皮细胞的方法.

关键词: 汗腺, 上皮细胞, 细胞,培养的

Abstract: Objective To rapidly isolate and culture sweat gland epithelial cells in vitro and to observe the characteristics of the cells.Methods The secretory coils of sweat glands were dissected and picked out under an anatomical microscope,then digested by collagenase.The harvested epithelial cells of sweat gland were observed for their growth characteristics and identified by immunohistochemistry.Results The cultured epithelial cells grew very well.About 3 weeks later,a distinctive cobblestone appearance was observed in the culture.The antibody-staining showed the cells were positive for epithelial membrane antigen and cytokeratin,but negative for actin,which confirmed that the cells were sweat gland epithelial cells.Conclusion A method is established for rapid isolation and culure of sweat gland epithelial cells in vitro.

Key words: Cells,cultured, Sweat glands, Epithelial cells