中华皮肤科杂志

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七种细胞角蛋白在皮肤上皮性肿瘤中的表达

孙莉1, 董艳光2, 徐志秀2, 刘淑梅3, 吴起嵩2, 李新功4   

  1. 1. 山东省东营市胜利油田中心医院皮肤科 257034;
    2. 山东省东营市胜利油田中心医院病理科 257034;
    3. 山东省东营市胜利油田中心医院检验科 257034;
    4. 东营市人民医院病理科
  • 收稿日期:2005-04-30 出版日期:2006-02-15 发布日期:2006-02-15

The expression of seven cytokeratins in skin epithelial tumors

SUN Li1, DONG Yan-guang2, XU Zhi-xiu2, LIU Shu-mei3, WU Qi-song2, LI Xin-gong4   

  1. Department of Dermatology, Shengli Oilfield Central Hospital, Dongying 257034, Shandong, China
  • Received:2005-04-30 Online:2006-02-15 Published:2006-02-15

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摘要： 目的 观察7种细胞角蛋白在皮肤上皮性肿瘤中的表达,并探讨其意义.方法 应用免疫组化S-P法对54例不同的皮肤上皮性肿瘤和20例正常皮肤进行细胞角蛋白7(K72.2)、细胞角蛋白8(C-51)、细胞角蛋白10(DE-K10)、细胞角蛋白14(LL002)、细胞角蛋白17(E3)、细胞角蛋白18(DC10)、细胞角蛋白19(KS19.1)标记,观察不同细胞角蛋白的表达.结果 54例皮肤上皮性肿瘤包括,鳞状细胞癌10例、基底细胞癌10例、毛发肿瘤19例、皮脂腺癌2例、汗腺肿瘤13例.皮肤上皮性肿瘤中7种细胞角蛋白的表达和分布有所不同.鳞状细胞癌、基底细胞癌和毛发分化的肿瘤中细胞角蛋白多数呈弥漫表达;而汗腺分化的肿瘤中,不同部位表达不同细胞角蛋白,每种肿瘤各有特点.结论 选择地检测一组细胞角蛋白的联合表达,有助于皮肤上皮性肿瘤的诊断和鉴别诊断.

关键词: 角蛋白, 肿瘤, 免疫组织化学, 试剂盒,诊断

Abstract: Objective To observe the expression of 7 cytokeratins in skin epithelial tumors and to assess their diagnostic value.Methods The expression of 7 cytokeratins,including cytokeratin 7(K72.2),cytokeratin 8(C-51),cytokeratin 10(DE-K10),cytokeratin 14(LL002),cytokeratin 17(E3),cytokeratin 18(DC10)and cytokeratin 19(KS19.1),was assessed by immunohistochemical staining (S-P method) in 54 cases of different skin epithelial tumors and 20 normal skin specimens.Results Of 54 cases studied,10 were squmous cell carcinoma (SCC),10 basal cell carcinoma (BCC),19 hair follicle tumor,2 sebaceous carcinoma,and 13 sweat gland tumor.The expression patterns and distribution of the 7 cytokeratins were different in different skin epithelial tumors.Most of the tumor cells stained diffusely for cytokeratins in SCC,BCC and hair follicle tumor;different cytokeratins expressed in different parts of each of the subtypes of sweat gland tumors.Conclusions Analysis of a selected group of cytokeratins may be helpful in the diagnosis and differential diagnosis of SCC,basal cell carcinoma and skin adenexal tumor.

Key words: Keratins, Neoplasms, Immunohistochemistry, Reagent kits,diagnostic