中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (1): 22-25.

• 论著 • 上一篇    下一篇

结缔组织生长因子mRNA在硬皮病模型小鼠真皮中的表达

廖朝晖1, 鲁建云1, 陈静1, 向亚平1, 左成忻1, 胡随瑜2   

  1. 1. 中南大学湘雅三医院皮肤科 长沙 410013;
    2. 中南大学湘雅医院中西医结合研究所
  • 收稿日期:2004-12-08 发布日期:2006-01-15
  • 基金资助:
    湖南省科技厅科研基金资助项目(02ssy3080)

Expression of CTGF mRNA in sclerotic skin of mice with bleomycin-induced scleroderma

LIAO Zhao-hui1, LU Jian-yun1, CHEN Jing1, XIANG Ya-ping1, ZUO Cheng-xin1, HU Sui-yu2   

  1. Department of Dermatology, the Third Xiangya Hospital, Central South University, Changsha 410013, China
  • Received:2004-12-08 Published:2006-01-15

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摘要： 目的 探讨博来霉素诱导硬皮病样改变.方法 24只BALB/c小鼠随机分为模型组和对照组,每日分别于背部皮下注射0.1mL博来霉素(300μg/mL)和0.1mL生理盐水,共4周.然后取背部皮肤行组织病理检查及真皮厚度测量,比色法测定羟脯氨酸含量,原位杂交法检测结缔组织生长因子(CTGF)mRNA表达细胞及RT-PCR法测定α1前胶原(pro-COL1A1)和CTGF mRNA表达量.结果 模型组小鼠皮肤表现出典型的硬皮病改变,其真皮较对照组显著增厚(P<0.01),羟脯氨酸含量显著升高(P<0.01);CTGF mRNA阳性杂交信号主要分布于真皮成纤维细胞胞浆中,模型组CTGF和pro-COL1A1 mRNA表达均较对照组显著增强(P分别<0.05和0.01);模型组皮肤羟脯氨酸含量与pro-COL1A1 mRNA表达量呈正相关(P<0.05),而pro-COL1A1 mRNA又与CTGF mRNA表达量呈正相关(P<0.01).结论 博来霉素可能通过上调成纤维细胞CTGF mRNA表达而增强pro-COL1A1 mRNA表达,最终导致皮肤胶原含量增多.

关键词: 硬皮病, 动物模型, CTGF, mRNA

Abstract: Objective To investigate the expression of connective tissue growth factor(CTGF) mRNA in the sclerotic skin of mice with bleomycin (BLM)-induced scleroderma.Methods Twenty-four SPF female BALB/c mice were randomly divided into the model group and the control group.The model mice were subcutaneously injected on the back with 0.1 mL of BLM (300 μg/mL,diluted in saline) daily for 4 weeks,while the controls were injected with saline in the same manner.After the final injection the skin section in the injected area was collected for histological examination,measurement of dermis thickness,determination of hydroxyproline contents by a colorimetric method,detection of CTGF mRNA expression by in situ hybridization and measurement of CTGF and pro-collagen α1(pro-COL1A1) mRNA expression by semiquantitative RT-PCR.Results Compared with the control mice,the model mice showed typical scleroderma histologically.The dermis was significantly thicker and the skin hydroxyproline content was significantly higher in the model group than that in the controls (both P<0.01).The positive signals for CTGF mRNA were mainly distributed in the cytoplasm of the fibroblasts in the dermis.The mRNA expression of both CTGF and pro-COL1A1 in the model mice was significantly up-regulated (P<0.05,P<0.01,respectively) than that in the controls.The hydroxyproline content in the skin of model mice correlated positively with the expression of pro-COL1A1 mRNA (P<0.05),meanwhile the latter correlated positively with the mRNA expression of CTGF (P<0.01).Conclusion BLM may increase the expression of pro-COL1A1 by up-regulating CTGF mRNA expression,which finally leads to the increase of the collagen content in the skin.

Key words: Scleroderma, Animal model, CTGF, mRNA