中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (1): 13-15.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者中Epstein-Barr病毒增殖期基因的表达

陈官芝1, 张炜2, 李惠3, 谭霞4, 罗兵5   

  1. 1. 青岛大学医学院附属医院皮肤科 266003;
    2. 青岛大学医学院附属医院检验科;
    3. 青岛大学医学院硕士研究生;
    4. 甘肃省金昌市金川集团公司医院皮肤科;
    5. 青岛大学医学院微生物学教研室
  • 收稿日期:2005-03-07 发布日期:2006-01-15

The correlation between the expression of Epstein-Barr virus lytic genes and systemic lupus erythematosus

CHEN Guan-zhi1, ZHANG Wei2, LI Hui3, TAN Xia4, LUO Bing5   

  1. Department of Dermatology, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China
  • Received:2005-03-07 Published:2006-01-15

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摘要： 目的 探讨EB病毒(EBV)增殖期基因表达与系统性红斑狼疮(SLE)的相关性.方法 PCR-Southern印迹检测44例SLE患者和43例正常对照外周血单一核细胞中EBV特异性DNA片段BamHⅠ-W,对EBV阳性标本进行RT-PCR和Southern印迹,检测即刻早期基因BZLF1、BRLF1,早期基因BARF1,晚期基因BcLF1和BLLF1的表达.结果 44例SLE患者血标本中有32例EBV阳性,正常对照组有3例EBV阳性,两组EBV阳性率差异有统计学意义(χ2=39.18,P<0.01),SLE活动期和稳定期患者EBV阳性率差异无统计学意义(q=0.40,P>0.05).32例EBV阳性SLE标本中2例BARF1mRNA阳性,均为活动期患者;14例BcLF1mRNA阳性,其中活动期11例,稳定期3例;均未检测到BZLF1,BRLF1和BLLF1表达;3例EBV阳性对照未检测到增殖期基因表达.结论 EBV感染与SLE有一定相关性,SLE中存在EBV增殖性感染,EBV在SLE中处于低水平活动状态.

关键词: 红斑狼疮,系统性, 基因,病毒, 疱疹病毒4型,人

Abstract: Objective To study the relationship between the expression of EBV lytic genes and systemic lupus erythematosus (SLE).Methods The blood samples from 44 SLE patients and 43 matched normal controls were tested for BamHⅠ-W,the specific DNA fragment of EBV,by polymerase chain reaction(PCR)-Southern analysis.RT-PCR and Southern blotting were used to detect the EBV lytic genes (including immediate early genes BZLF1 and BRLF1,early genes BARF1,late genes BcLF1 and BLLF1) in EBV DNA-positive SLE patients.Results The EBV DNA was positive in 32 SLE patients and 3 controls.There was significant difference between the SLE patients and controls in the EBV DNA expression (χ2=39.18,P<0.01),while no difference was observed between patients with active SLE and those with inactive SLE (q=0.40,P>0.05).In the EBV DNA-positive SLE patients,2 (both with active SLE) were positive for BARF1 mRNA;14 (11 with active SLE and 3 with inactive SLE) were positive for BcLF1 mRNA;none was positive for BZLF1,BRLF1 or BLLF1 mRNA.No lytic genes were expressed in any of the 3 EBV DNA-positive controls.Conclusion EBV infection may be related to the development of SLE.EBV lytic infection exists in some SLE patients.EBV is at a low level of lytic activity in patients with SLE.

Key words: Lupus erythematosus,systemic, Herpesvirus 4,human, Genes,viral