中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (11): 621-624.

• 论著 •    下一篇

基底细胞癌和鳞状细胞癌中转化生长因子βⅠ型和Ⅱ型受体表达

李颖1, 何威1, 阎国富1, 何云志1, 黄海1, 姚磊2, 林自华1, 吴军1   

  1. 1. 第三军医大学新桥医院皮肤科 重庆 400073;
    2. 第三军医大学新桥医院检验科 重庆 400073
  • 收稿日期:2005-12-12 出版日期:2006-11-15 发布日期:2006-11-15
  • 通讯作者: 何威, email:weihegj@yahoo.com E-mail:email;weihegj@yahoo.com
  • 作者简介:
  • 基金资助:
    国家自然科学基金资助项目(30371298)

Down-regulation of TGF β receptor expression in human basal cell carcinoma and squamous cell carcinoma

LI Ying1, HE Wei1, YAN Guo-fu1, HE Yun-zhi1, HUANG Hai1, YAO Lei2, LIN Zi-hua1, WU Jun1   

  1. Department of Dermatology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China
  • Received:2005-12-12 Online:2006-11-15 Published:2006-11-15

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摘要： 目的探讨转化生长因子β(TGFβ)受体在基底细胞癌和鳞状细胞癌中表达水平的变化及其意义。方法采用实时定量PCR和SP免疫组化技术分别检测基底细胞癌、鳞状细胞癌及正常人对照皮肤中Ⅰ型和Ⅱ型TGFβ受体(TGFβRⅠ,TGFβRⅡ)mRNA和蛋白的表达。结果对18例基底细胞癌、24例鳞状细胞癌患者的皮损及正常人对照皮肤的研究显示,基底细胞癌和鳞状细胞癌皮损TGFβRⅠ和TGFβRⅡ的mRNA表达水平均显著低于正常人对照皮肤。免疫组化试验结果显示;TGFβRⅠ染色强度在基底细胞癌组、鳞状细胞癌组较正常人对照皮肤组显著降低(P<0.001);TGFβRⅡ在二组表皮肿瘤与正常人对照皮肤组问表达差异也有统计学意义(P<0.01)。结论基底细胞癌和鳞状细胞癌中TGFβ受体表达下调可能有助于这些上皮细胞起源的表皮肿瘤的形成。

关键词: 癌,基底细胞, 癌,鳞状细胞, 转化生长因子β, 受体,转化生长因子β

Abstract: Objective To investigate the expression and significance of transforming growth factor beta receptors (TGFβRⅠ,TGFβRⅡ) in basal cell carcinoma and squamous cell carcinoma.Methods The expression of TGFβRⅠand TGFβRⅡwere assessed by quantitative real time PCR and streptavidin-peroxidase (SP) immunohistochemical techniques in specimens of basal cell carcinoma,squamous cell carcinoma and normal control skin.Results Eighteen patients with basal cell carcinoma and twenty-four with squamous cell carcinoma were enrolled in the study.The expression levels of TGFβRⅠand RⅡmRNA were significantly down-regulated in basal cell carcinoma and squamous cell carcinoma in comparison with those in control skin specimens (P<0.001).The intensity of positive immunostaining for TGFβRⅠand TGFβRⅡwere also significantly down-regulated in basal cell carcinoma and squamous cell carcinoma in comparison with those in normal skin (P< 0.01).Conclusions Down-regulation of TGFβreceptor expression may interfere with TGFβsignaling transduction pathway,and contribute to the formation of basal cell carcinoma and squamous cell carcinoma.

Key words: Carcinoma, basal cell, Carcinoma, squamous cell, Transforming growth factor beta, Receptors, transforming growth factor beta