中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (2): 112-114.

• 论著 • 上一篇    下一篇

疣状瓶霉致皮肤着色真菌病一例及致病菌的辅酶Q和DNA序列测定

周冼苡1, Paride Abliz2, 龙泽香代子2, 席丽艳1   

  1. 1. 南方医科大学南方医院皮肤科 广州 510515;
    2. Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan
  • 收稿日期:2004-02-22 出版日期:2005-02-15 发布日期:2005-02-15

Chromomycosis Caused by Phialophora Verrucosa:a Case Report, and Ubiquinone System and DNA Sequence Analysis of Pathogen

Zhou Xian-yi1, Paride Abliz2, Kayoko Takizawa2, XI Li-yan1   

  1. Department of Dermatology, Nanfang Hospital, Nanfang Medical University, Guangzhou 510515, China
  • Received:2004-02-22 Online:2005-02-15 Published:2005-02-15

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摘要： 目的 报告1例疣状瓶霉致皮肤着色真菌病,并探讨其致病菌的实验室特征。方法 皮损组织病理学检查、真菌学检查,分离株辅酶Q检测、DNA序列鉴定。结果 该病表现为慢性疣状增殖性斑块,病程长,外伤后易发病。真皮层可见棕色厚壁孢子。菌落生长缓慢,需观察4周。小培养沙氏琼脂培养基无特殊结构,马铃薯葡萄糖琼脂见瓶梗多生长于短侧枝或气生菌丝顶端,玉米粉琼脂Ⅰ见瓶梗多侧生于菌丝,后两者其他结构相同。辅酶Q系统检测及LSUrDNA D1/D2区域碱基序列测定,与标准菌株比较完全相同。结论 我国该病较少见,确诊需作真菌培养及组织病理,应同时采用沙氏琼脂培养基、马铃薯葡萄糖琼脂、玉米粉琼脂Ⅰ3种培养基培养。辅酶Q检测及分子生物学鉴定可排除表型不同的干扰,有利于进一步鉴定菌种。

关键词: 瓶霉菌属, 着色真菌病, 辅酶Q, 序列分析, DNA

Abstract: Objective To report a case of chromomycosis caused by Phialophora verrucosa and explore the laboratory features of the pathogen. Methods Skin lesion was examined by histopathology and fungus culture. The morphology of the isolate was observed by microscopy and scanning electron microscopy. The coenzyme Q system of this isolate was analyzed by HPLC assay. The DNA sequences of LSU rDNA D1/D2 region of this isolate and a standard fungus strain were compared. Results The initial lesion was an erythematous papule that subsequently developed into one or multiple coalescing warty papules or plaques slowly. The bronze-colored spores could were observed in the dermis or macrophages. The isolate grew very slowly, requiring 4 weeks of incubation. Microscopically, no characteristic structures were found on Sabourand's dextrose agar, while there were vase-like structures, which were referred to as phialides on potato dextrose agar (PDA) and corn meal agar I (CMA-I). The phialides on PDA mostly grew at the top of hypha, but on CMA-I they mostly grew on the side of hypha. The isolate contained coenzyme Q-10, and its DNA sequence of LSU rDNA D1/D2 region completely consistent with those of the standard strain. Conclusion Chromomycosis caused by Phialophora verrucosa is rare in China. It can be diagnosed by fungus culture and histopathological examination. Coenzyme Q system analysis and DNA sequencing can exclude the interference from different phenotypes.

Key words: Phialophora, Chromoblastomycosis, Ubiquinone, Sequence analysis, DNA