中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (2): 92-94.

• 论著 • 上一篇    下一篇

中药对豚鼠皮肤酪氨酸酶mRNA水平的影响及致色素作用

马景昕1, 涂彩霞1, 陈晓燕2, 张开立2, 刘佳2, 李宏2   

  1. 1. 大连医科大学附属第二医院皮肤科 116027;
    2. 大连医科大学基础医学院细胞生物和遗传学实验室 116027
  • 收稿日期:2004-01-05 出版日期:2005-02-15 发布日期:2005-02-15
  • 基金资助:
    辽宁省自然科学基金资助项目(972290)

Effects of Ethanol Extracts of Several Traditional Chinese Medicinal Herbs on Tyrosinase Expression and Melanogenesis in Guinea Pig Skin

MA Jing-xin1, TU Cai-xia1, CHEN Xiao-yan2, ZHANG Kai-li2, LIU Jia2, LI Hong2   

  1. Department of Dermatology, 2nd Affiliated Hospital of Dalian Medical University, Dalian 116027, China
  • Received:2004-01-05 Online:2005-02-15 Published:2005-02-15

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摘要： 目的 探讨中药对皮肤酪氨酸酶基因表达、色素生成和黑素细胞增殖的影响。方法 选择在离体实验中对酪氨酸酶有激活作用的旱莲草等7种中药,以棕色豚鼠为动物模型,采用mRNA原位杂交观察中药外涂对豚鼠皮肤酪氨酸酶mRNA水平的影响,并用Schmorl法染色和多巴-氧化酶染色观察中药促色素生成及黑素细胞增殖的作用。结果 原位杂交显示中药处理组的酪氨酸酶阳性细胞数和杂交信号明显高于对照组(P<0.01),尤其以旱莲草、夏枯草、刺蒺藜等显著。Schmorl法和多巴-氧化酶染色示7种中药能使豚鼠表皮基底层内含有黑素颗粒的细胞数和多巴阳性细胞的数量增多(P<0.05),但7种中药的两种作用并不完全平行(P>0.1)。结论 旱莲草等7种中药均具有上调豚鼠皮肤酪氨酸酶的基因表达、促进黑素生成和黑素细胞增殖的作用,提示中药的作用不仅限于转录水平,有可能影响黑素细胞的增殖和黑素颗粒的生成。

关键词: 中草药, 一元酚单氧酶, 黑素类

Abstract: Objective To explore the effects of traditional Chinese medicinal herbs (TCMHs) on the expression of tyrosinase gene, melanogenesis and proliferation of melanocytes and elucidate the mechanism of TCMHs in promoting melanogenesis. Methods Seven TCMHs including Herba Ecliptae, Spica Prunellae, Caulis Spatholob, etc, which were known to be effective in activating tyrosinase in vivo, were selected. Brownish guinea pigs were selected as the experimental model. The mRNA in situ hybridization (ISH), Schmorl-staining and dopa-oxygenase staining were performed to observe the effects of TCMHs on gene expression of tyrosinase, melanogenesis and melanocyte proliferation. Results The mRNA ISH showed that these seven drugs, especially Herba Ecliptae,Spica Prunellae and Tribulus terrestris could significantly increase the number of positive cells and the intensity of hybridization signal in the treated group as compared with that in the control group (P<0.01). In Schmorl staining and dopa-oxygenase staining, the number of cells containing melanin granules and dopa-staining positive cells per 100 basal layer cells were significantly increased in TCM treated groups (P<0.05), and these two effects of TCMHs were not parallel with each other (P>0.1). Conclusions These results suggested that these 7 TCMHs including Herba Ecliptae can upregulate the gene expression of tyrosinase, enhance the melanogenesis and promote the proliferation of melanocytes.

Key words: Drugs, Chinese herbal, Monophenol monooxygenase, Melanins